TY - JOUR
T1 - Identification of genetic polymorphisms through comparative DNA sequence analysis on the K-ras gene
T2 - Implications for lung tumor susceptibility
AU - Wang, Min
AU - Wang, Yian
AU - You, Ming
N1 - Funding Information:
Received 16 March 2004; accepted 3 April 2004. This work has been supported in part by NIH Grants R01CA099147 and R01CA58554. Address correspondence to Ming You, Department of Surgery and The Alvin J. Siteman Cancer Center, Campus Box 8109, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
PY - 2005/3
Y1 - 2005/3
N2 - In the present study, the authors performed a comparative DNA sequence analysis of the K-ras gene. By comparing sequences from different mouse inbred strains, the authors have identified new nucleotide polymorphisms in the noncoding regions of mouse K-ras gene. They have also identified noncoding DNA segments evolutionary conserved among the human, mouse, and rat. Computational analysis for transcription factor binding sites suggests that these polymorphic and conserved DNA sequences harbor potential cis-regulatory elements, which may contribute to the transcriptional regulation of the K-ras gene. Further studies on these potential regulatory sites may help to elucidate the fundamental mechanism underlying allele-specific activation and expression of K-ras gene in hybrid mouse lung tumors, which determines lung tumor susceptibility in mice.
AB - In the present study, the authors performed a comparative DNA sequence analysis of the K-ras gene. By comparing sequences from different mouse inbred strains, the authors have identified new nucleotide polymorphisms in the noncoding regions of mouse K-ras gene. They have also identified noncoding DNA segments evolutionary conserved among the human, mouse, and rat. Computational analysis for transcription factor binding sites suggests that these polymorphic and conserved DNA sequences harbor potential cis-regulatory elements, which may contribute to the transcriptional regulation of the K-ras gene. Further studies on these potential regulatory sites may help to elucidate the fundamental mechanism underlying allele-specific activation and expression of K-ras gene in hybrid mouse lung tumors, which determines lung tumor susceptibility in mice.
KW - Comparative DNA sequence analysis
KW - K-ras
KW - Lung tumor susceptibility
KW - Mouse
KW - Transcription factor binding sites
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U2 - 10.1080/01902140490495543
DO - 10.1080/01902140490495543
M3 - Article
C2 - 15824019
AN - SCOPUS:14644388822
SN - 0190-2148
VL - 31
SP - 165
EP - 177
JO - Experimental Lung Research
JF - Experimental Lung Research
IS - 2
ER -