Identification of Evi-3, a novel common site of retroviral integration in mouse AKXD B-cell lymphomas

Monica J. Justice, Herbert C. Morse, Nancy A. Jenkins, Neal G. Copeland

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

We have identified a novel common site of ecotropic viral integration called ecotropic viral integration site 3 (Evi-3) in B-cell lineage lymphomas of the AKXD recombinant inbred strains of mice. A number of virally induced pre-B-, B-, myeloid, and T-cell lymphomas were screened for viral rearrangements at Evi-3; rearrangements were found in pre-B- and B-cell lymphomas but not in other hematopoietic tumors. Genetic mapping studies localized Evi-3 to mouse chromosome 18, distinct from proto-oncogene and common viral integration loci identified previously in the mouse. Each proviral integration at Evi-3 is contained within a 200-bp region that lies inside a CpG island. All but one of the proviruses have integrated in the same 5'-to-3' transcriptional orientation. Transcripts from Evi-3 are expressed in a developmentally regulated manner in B cells. Taken together, these data suggest that Evi-3 represents a novel proto-oncogene involved in mouse B-cell lymphomas.

Original languageEnglish (US)
Pages (from-to)1293-1300
Number of pages8
JournalJournal of virology
Volume68
Issue number3
DOIs
StatePublished - Mar 1994

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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