TY - JOUR
T1 - Identification of estrogen-regulated genes of potential importance for the regulation of trabecular bone mineral density
AU - Lindberg, Marie K.
AU - Movérare, Sofia
AU - Eriksson, Anna Lena
AU - Skrtic, Stanko
AU - Gao, Hui
AU - Dahlman-Wright, Karin
AU - Gustafsson, Jan Åke
AU - Ohlsson, Claes
PY - 2002/12/1
Y1 - 2002/12/1
N2 - Estrogen is of importance for the regulation of trabecular bone mineral density (BMD). The aim of this study was to search for possible mechanisms of action of estrogen on bone. Ovariectomized (OVX) mice were treated with 17β-estradiol. Possible effects of estrogen on the expression of 125 different bone-related genes in humerus were analyzed using the microarray technique. Estrogen regulated 12 of these genes, namely, two growth factor-related genes, 8 cytokines, and 2 bone matrix-related genes. Five of the 12 genes are known to be estrogen-regulated, and the remaining 7 genes are novel estrogen-regulated genes. Seven genes, including interleukin-1 receptor antagonist (IL-1ra), IL-1receptor type II (IL-1RII), insulin-like growth factor-binding protein 4 (IGFBP-4), transforming growth factor β (TGF-β), granulocyte colony-stimulating factor receptor (G-CSFR), leukemia inhibitory factor receptor (LIFR), and soluble IL-4 receptor (sIL-4R) were selected as probable candidate genes for the trabecular bone-sparing effect of estrogen, as the mRNA levels of these genes were highly correlated (r2 > 0.65) to the trabecular BMD. The regulation of most of these seven genes was predominantly estrogen receptor α (ER-α)-mediated (5/7) while some genes (2/7) were regulated both via ER-α and ER-β. In conclusion, by using the microarray technique, we have identified four previously known and three novel estrogen-regulated genes of potential importance for the trabecular bone-sparing effect of estrogen.
AB - Estrogen is of importance for the regulation of trabecular bone mineral density (BMD). The aim of this study was to search for possible mechanisms of action of estrogen on bone. Ovariectomized (OVX) mice were treated with 17β-estradiol. Possible effects of estrogen on the expression of 125 different bone-related genes in humerus were analyzed using the microarray technique. Estrogen regulated 12 of these genes, namely, two growth factor-related genes, 8 cytokines, and 2 bone matrix-related genes. Five of the 12 genes are known to be estrogen-regulated, and the remaining 7 genes are novel estrogen-regulated genes. Seven genes, including interleukin-1 receptor antagonist (IL-1ra), IL-1receptor type II (IL-1RII), insulin-like growth factor-binding protein 4 (IGFBP-4), transforming growth factor β (TGF-β), granulocyte colony-stimulating factor receptor (G-CSFR), leukemia inhibitory factor receptor (LIFR), and soluble IL-4 receptor (sIL-4R) were selected as probable candidate genes for the trabecular bone-sparing effect of estrogen, as the mRNA levels of these genes were highly correlated (r2 > 0.65) to the trabecular BMD. The regulation of most of these seven genes was predominantly estrogen receptor α (ER-α)-mediated (5/7) while some genes (2/7) were regulated both via ER-α and ER-β. In conclusion, by using the microarray technique, we have identified four previously known and three novel estrogen-regulated genes of potential importance for the trabecular bone-sparing effect of estrogen.
KW - Estrogen
KW - Insulin-like growth factor binding protein 4
KW - Interleukin-1
KW - Leukemia inhibitory factor receptor
KW - Microarray
KW - Trabecular bone mineral density
KW - Transforming growth factor β
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U2 - 10.1359/jbmr.2002.17.12.2183
DO - 10.1359/jbmr.2002.17.12.2183
M3 - Article
C2 - 12469912
AN - SCOPUS:0344211932
SN - 0884-0431
VL - 17
SP - 2183
EP - 2195
JO - Journal of Bone and Mineral Research
JF - Journal of Bone and Mineral Research
IS - 12
ER -