TY - JOUR
T1 - Identification of environmental chemicals that induce yolk malabsorption in zebrafish using automated image segmentation
AU - Kalasekar, Sharanya Maanasi
AU - Zacharia, Eleni
AU - Kessler, Noah
AU - Ducharme, Nicole A.
AU - Gustafsson, Jan-Ake
AU - Kakadiaris, Ioannis A.
AU - Bondesson, Maria
N1 - Funding Information:
This study was funded by grants from the Environmental Protection Agency ( R834289 ), the National Institute of Health/National Institute of Environmental Health Sciences ( R21ES020036 ), and in part by a training fellowship from the Keck Center Computational Cancer Biology Training Program of the Gulf Coast Consortia (CPRIT Grant No. RP101489 ). JÅG is grateful for a grant from the Robert A. Welch Foundation ( E-0004 ). The views expressed in the article do not necessarily reflect the views of the funders.
Funding Information:
We would like to thank Dr. Kawakami, the National BioResource Project from the Ministry of Education, Culture, Sports, Science and Technology of Japan for the transgenic fish HGn50D.
Publisher Copyright:
© 2014 Elsevier Inc.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - Environmental factors affecting nutrient availability during development can cause predisposition to diseases later in life. To identify chemicals in the environment capable of altering nutrient mobilization, we analyzed yolk malabsorption in the zebrafish embryo, which relies on maternally-derived yolk for nutrition during its first week of life. Embryos of the transgenic zebrafish line HGn50D, which fluoresce in the yolk syncytial layer, were exposed from two to five days post fertilization to different chemicals. We developed a software package to automatically and accurately segment and quantify the area of the fluorescing yolk in images captured at the end of the treatment period. Based on this quantification, we found that prochloraz decreased yolk absorption, while butralin, tetrabromobisphenol A, tetrachlorobisphenol A and tributyltin increased yolk absorption. Given the number and variety of industrial chemicals in commerce today, development of automated image processing to perform high-speed quantitative analysis of biological effects is an important step for enabling high throughput screening to identify chemicals altering nutrient absorption.
AB - Environmental factors affecting nutrient availability during development can cause predisposition to diseases later in life. To identify chemicals in the environment capable of altering nutrient mobilization, we analyzed yolk malabsorption in the zebrafish embryo, which relies on maternally-derived yolk for nutrition during its first week of life. Embryos of the transgenic zebrafish line HGn50D, which fluoresce in the yolk syncytial layer, were exposed from two to five days post fertilization to different chemicals. We developed a software package to automatically and accurately segment and quantify the area of the fluorescing yolk in images captured at the end of the treatment period. Based on this quantification, we found that prochloraz decreased yolk absorption, while butralin, tetrabromobisphenol A, tetrachlorobisphenol A and tributyltin increased yolk absorption. Given the number and variety of industrial chemicals in commerce today, development of automated image processing to perform high-speed quantitative analysis of biological effects is an important step for enabling high throughput screening to identify chemicals altering nutrient absorption.
KW - High throughput screening
KW - Image analysis
KW - Toxicity
KW - Yolk
KW - Zebrafish
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U2 - 10.1016/j.reprotox.2014.10.022
DO - 10.1016/j.reprotox.2014.10.022
M3 - Article
C2 - 25462786
AN - SCOPUS:84939416137
VL - 55
SP - 20
EP - 29
JO - Reproductive Toxicology
JF - Reproductive Toxicology
SN - 0890-6238
ER -