Identification of early tolerance regulator genes induced by allochimeric therapy using microarray-based genomewide scan

D. Liu, X. D. Shen, Z. Fang, F. Gao, N. Semiletova, M. J. Cao, R. W. Busuttil, J. W. Kupiec-Weglinski, R. M. Ghobrial

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

We have demonstrated that peri- or postoperative delivery of allochimeric [a1h u]-RT1.Aa class I major histocompatibility complex molecules with donor-type (RT1Au) immunogenic epitopes presented in recipient-type (RT1Aa) sequences induced donor-specific tolerance in ACI (RT1a) recipients of WF (RT1u) heart allografts. A genomic scan during the early posttransplant period was performed to elucidate the underlying operative mechanisms. A rat genome study after transplantation was carefully designed using Affymetrix Rat Genome 230 2.0 Array. The allochimeric treatment group is 3-day cyclosporine (CsA)-treated ACI recipients that accepted Wistar Furth RT1u cardiac allografts with postoperative dosage of allochimeric molecules, while the control is 3-day CsA-treated ACI recipients of WF cardiac allografts. All the samples were harvested 5 days after heart transplant as the early stage of tolerance detection. Following array data normalization and modeling, we compared the above two treatment groups and identified a total of 250 tolerance regulator genes induced by allochimeric molecules only.

Original languageEnglish (US)
Pages (from-to)1942-1943
Number of pages2
JournalTransplantation Proceedings
Volume37
Issue number4
DOIs
StatePublished - May 2005

ASJC Scopus subject areas

  • Surgery
  • Transplantation

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