TY - JOUR
T1 - Identification of a novel Sry-related gene and its germ cell-specific expression
AU - Osaki, Emiko
AU - Nishina, Yukio
AU - Inazawa, Johji
AU - Copeland, Neal G.
AU - Gilbert, Debra J.
AU - Jenkins, Nancy A.
AU - Ohsugi, Miho
AU - Tezuka, Tohru
AU - Yoshida, Mitsuaki
AU - Semba, Kentaro
N1 - Funding Information:
We thank H. Hirai, T. Suzuki, J. Fujimoto (IMSUT), N. Takamatsu (Kitasato University) and T. Akiyama (University of Tokyo) for materials and helpful discussions, Y. Nishimune (Osaka University) for jsd/jsd mice, P. Bartel, S. Fields and S. Hollenberg for the yeast two hybrid system and A. Reuss for excellent technical assistance. This research was supported in part by the Uehara Memorial Foundation and the National Cancer Institute, DHHS, under a contract with ABL.
PY - 1999/6/15
Y1 - 1999/6/15
N2 - Sox family proteins are characterized by a unique DNA-binding domain, a HMG box which shows at least 50% sequence similarity with mouse Sry, the sex-determining factor. At present almost 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development, Some are involved in human diseases, including sex reversal. Here we report the isolation of a novel member of the Sox gene family, Sox30, which may constitute a distinct subgroup of this family. Using a bacterially expressed DNA-binding domain of Sox30, we show that it is able to specifically recognize the ACAAT motif. Furthermore, Sox30 is capable of activating transcription from a synthetic promoter containing the ACAAT motif. The specific expression of Sox30 in normal testes, but not in maturing germ cell-deficient testes, suggests the involvement of Sox30 in differentiation of male germ cells. Mapping analyses revealed that the Sox30 gene is located on human chromosome 5 (5q33) and on mouse chromosome 11.
AB - Sox family proteins are characterized by a unique DNA-binding domain, a HMG box which shows at least 50% sequence similarity with mouse Sry, the sex-determining factor. At present almost 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development, Some are involved in human diseases, including sex reversal. Here we report the isolation of a novel member of the Sox gene family, Sox30, which may constitute a distinct subgroup of this family. Using a bacterially expressed DNA-binding domain of Sox30, we show that it is able to specifically recognize the ACAAT motif. Furthermore, Sox30 is capable of activating transcription from a synthetic promoter containing the ACAAT motif. The specific expression of Sox30 in normal testes, but not in maturing germ cell-deficient testes, suggests the involvement of Sox30 in differentiation of male germ cells. Mapping analyses revealed that the Sox30 gene is located on human chromosome 5 (5q33) and on mouse chromosome 11.
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U2 - 10.1093/nar/27.12.2503
DO - 10.1093/nar/27.12.2503
M3 - Article
C2 - 10359848
AN - SCOPUS:0033564791
SN - 0305-1048
VL - 27
SP - 2503
EP - 2510
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 12
ER -