Identification of a novel NLS of herpes simplex virus type 1 (HSV-1) VP19C and its nuclear localization is required for efficient production of HSV-1

You Li, Lei Zhao, Shuai Wang, Junji Xing, Chunfu Zheng

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Herpes simplex virus type 1 (HSV-1) triplex is a complex of three protein subunits, consisting of two copies of VP23 and one copy of VP19C. Here, we identified a non-classical NLS of VP19C between aa 50 and 61, and the nuclear import of VP19C was mediated by RanGTP and importin β1-, but not importin α5-, dependent pathway. Additionally, recombinant virus harbouring this NLS mutation (NLSm) replicates less efficiently as wild-type. These data strongly suggested that the nuclear import of VP19C is required for efficient HSV-1 production.

Original languageEnglish (US)
Pages (from-to)1869-1875
Number of pages7
JournalJournal of General Virology
Volume93
Issue numberPART 9
DOIs
StatePublished - Sep 2012

ASJC Scopus subject areas

  • Virology

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