TY - JOUR
T1 - Identification of a novel NLS of herpes simplex virus type 1 (HSV-1) VP19C and its nuclear localization is required for efficient production of HSV-1
AU - Li, You
AU - Zhao, Lei
AU - Wang, Shuai
AU - Xing, Junji
AU - Zheng, Chunfu
PY - 2012/9
Y1 - 2012/9
N2 - Herpes simplex virus type 1 (HSV-1) triplex is a complex of three protein subunits, consisting of two copies of VP23 and one copy of VP19C. Here, we identified a non-classical NLS of VP19C between aa 50 and 61, and the nuclear import of VP19C was mediated by RanGTP and importin β1-, but not importin α5-, dependent pathway. Additionally, recombinant virus harbouring this NLS mutation (NLSm) replicates less efficiently as wild-type. These data strongly suggested that the nuclear import of VP19C is required for efficient HSV-1 production.
AB - Herpes simplex virus type 1 (HSV-1) triplex is a complex of three protein subunits, consisting of two copies of VP23 and one copy of VP19C. Here, we identified a non-classical NLS of VP19C between aa 50 and 61, and the nuclear import of VP19C was mediated by RanGTP and importin β1-, but not importin α5-, dependent pathway. Additionally, recombinant virus harbouring this NLS mutation (NLSm) replicates less efficiently as wild-type. These data strongly suggested that the nuclear import of VP19C is required for efficient HSV-1 production.
UR - http://www.scopus.com/inward/record.url?scp=84865126859&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84865126859&partnerID=8YFLogxK
U2 - 10.1099/vir.0.042697-0
DO - 10.1099/vir.0.042697-0
M3 - Article
C2 - 22622329
AN - SCOPUS:84865126859
SN - 0022-1317
VL - 93
SP - 1869
EP - 1875
JO - Journal of General Virology
JF - Journal of General Virology
IS - PART 9
ER -