TY - JOUR
T1 - Identification of a novel member of the nuclear receptor superfamily which is closely related to rev-erba
AU - Enmark, Eva
AU - Kainu, Tommi
AU - Pelto-Huikko, Markku
AU - Gustafsson, Jan Åke
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1994/10/15
Y1 - 1994/10/15
N2 - Degenerate PCR primers were designed based upon conserved regions within the DNA and ligand binding domains of several nuclear receptors. PCR was performed using these primers starting with total brain cDNA. Several members of the nuclear receptor superfamily were identified, one of which was novel, but showing a high degree of homology to the previously known orphan receptor Rev-ErbA. This new orphan receptor, Rev-ErbAβ, was further characterised, and a cDNA with an intact open reading frame was isolated from a rat brain cDNA library. Northern blot analysis shows two different sizes of mRNA, 4 and 5.5 kb, respectively, in all tissues analysed. A more detailed study of this orphan was then performed using in situ hybridisation, showing a high expression of the new orphan receptor in particular in the cerebellum, the dentate gyrus of the hippocampus and pituitary gland of adult rats.
AB - Degenerate PCR primers were designed based upon conserved regions within the DNA and ligand binding domains of several nuclear receptors. PCR was performed using these primers starting with total brain cDNA. Several members of the nuclear receptor superfamily were identified, one of which was novel, but showing a high degree of homology to the previously known orphan receptor Rev-ErbA. This new orphan receptor, Rev-ErbAβ, was further characterised, and a cDNA with an intact open reading frame was isolated from a rat brain cDNA library. Northern blot analysis shows two different sizes of mRNA, 4 and 5.5 kb, respectively, in all tissues analysed. A more detailed study of this orphan was then performed using in situ hybridisation, showing a high expression of the new orphan receptor in particular in the cerebellum, the dentate gyrus of the hippocampus and pituitary gland of adult rats.
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U2 - 10.1006/bbrc.1994.2424
DO - 10.1006/bbrc.1994.2424
M3 - Article
C2 - 7945391
AN - SCOPUS:0028097504
SN - 0006-291X
VL - 204
SP - 49
EP - 56
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -