Identification of a novel chemokine (CCL28), which binds CCR10 (GPR2)

Wei Wang, Hortensia Soto, Elizabeth R. Oldham, Matthew E. Buchanan, Bernhard Homey, Daniel Catron, Nancy Jenkins, Neal G. Copeland, Debra J. Gilbert, Nhung Nguyen, John Abrams, David Kershenovich, Kathleen Smith, Terri McClanahan, Alain P. Vicari, Albert Zlotnik

Research output: Contribution to journalArticle

215 Scopus citations

Abstract

We report the identification and characterization of a novel CC chemokine designated CCL28 and its receptor CCR10, known previously as orphan G-protein-coupled receptor GPR2. Human and mouse CCL28 share 83% identity at the amino acid and 76% at the nucleic acid levels. We also identified the mouse homologues of CCL28 and of CCR10, which map to mouse chromosomes 13 and 11, respectively. CCL28 is expressed in a variety of human and mouse tissues, and it appears to be predominantly produced by epithelial cells. Both human and mouse CCL28 induce calcium mobilization in human and mouse CCR10-expressing transfectants. CCL28 desensitized the calcium mobilization induced in CCR10 transfectants by CCL27, indicating that these chemokines share this new chemokine receptor. In vitro, recombinant human CCL28 displays chemotactic activity for resting CD4 or CD8 T cells.

Original languageEnglish (US)
Pages (from-to)22313-22323
Number of pages11
JournalJournal of Biological Chemistry
Volume275
Issue number29
DOIs
StatePublished - Jul 21 2000

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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