@article{9a8c0153e8e84d02a34feef21cdbee2d,
title = "Identification of a MicroRNA that Activates Gene Expression by Repressing Nonsense-Mediated RNA Decay",
abstract = "Nonsense-mediated decay (NMD) degrades both normal and aberrant transcripts harboring stop codons in particular contexts. Mutations that perturb NMD cause neurological disorders in humans, suggesting that NMD has roles in the brain. Here, we identify a brain-specific microRNA-miR-128-that represses NMD and thereby controls batteries of transcripts in neural cells. miR-128 represses NMD by targeting the RNA helicase UPF1 and the exon-junction complex core component MLN51. The ability of miR-128 to regulate NMD is a conserved response occurring in frogs, chickens, and mammals. miR-128 levels are dramatically increased in differentiating neuronal cells and during brain development, leading to repressed NMD and upregulation of mRNAs normally targeted for decay by NMD; overrepresented are those encoding proteins controlling neuron development and function. Together, these results suggest the existence of a conserved RNA circuit linking the microRNA and NMD pathways that induces cell type-specific transcripts during development.",
author = "Bruno, {Ivone G.} and Rachid Karam and Lulu Huang and Anjana Bhardwaj and Lou, {Chih H.} and Shum, {Eleen Y.} and Song, {Hye Won} and Corbett, {Mark A.} and Gifford, {Wesley D.} and Jozef Gecz and Pfaff, {Samuel L.} and Wilkinson, {Miles F.}",
note = "Funding Information: We are grateful to Jens Lykke-Anderson (UCSD) and Catherine Tomasseto (Institut de Genetique, Strasbourg, France) for expression vectors and antiserum. We thank Kim Tolias (Baylor College of Medicine) and Sadhan Majumder (M.D. Anderson Cancer Center) for providing primary cells; Howard Gutstein (M.D. Anderson Cancer Center) for providing rat brain tissue sections; Christopher Kinter (Salk Institute) for providing X. laevis eggs; Benjamin Yu and Christopher Cowing-Zitron (UCSD) for bioinformatic support; and Gilbert Cote, Angela Bhalla, and Miriam Buttigieg (M.D. Anderson Cancer Center) for their experimental assistance and/or intellectual contributions to various portions of this manuscript. Finally, we thank Fred “Rusty” Gage, Ahmed Denli, and Leah Boyer (Salk Institute, La Jolla) for antibodies and helpful advice. This work was supported by National Institutes of Health grant GM058595. ",
year = "2011",
month = may,
day = "20",
doi = "10.1016/j.molcel.2011.04.018",
language = "English (US)",
volume = "42",
pages = "500--510",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "4",
}