Identification of a detergent-solubilized plasma membrane protein from rat liver recognizing apolipoprotein A-IV

Giancarlo Ghiselli, William Crump, Roberto Musanti, Antonio Gotto

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Apolipoprotein A-IV (apo A-IV) is present in plasma associated to both HDL and as a complex with lipids that cannot be flotated by ultracentrifugation at 1.21 g/ml density. Apo A-IV is likely an important molecular determinant in HDL binding to the liver. In this communication, data are presented supporting the view that a specific liver plasma membrane protein of Mr 95000 is a constituent of the apo A-IV binding site. The protein was solubilized with CHAPS from purified rat liver plasma membranes and subjected to SDS-PAGE. Transblotted to nitrocellulose sheet could be identified as recognizing 125I-apo A-IV-DMPC by autoradiography. 125I-apo A-I-DMPC and radioiodinated rat apo E-poor HDL, also bound to the protein. Apo B-100 (as human LDL) and apo C-III did not bind. The protein identified is likely to be the same that has been previously identified by Graham and Oram ((1987) J. Biol. Chem. 262, 7439-7442) as 'HDL receptor protein'.

Original languageEnglish (US)
Pages (from-to)173-175
Number of pages3
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume1044
Issue number1
DOIs
StatePublished - May 1 1990

Keywords

  • (Rat liver)
  • Apolipoprotein A-IV
  • Cholesterol
  • Lipid metabolism
  • Membrane protein
  • Receptor
  • Reverse cholesterol transport

ASJC Scopus subject areas

  • Endocrinology
  • Biophysics
  • Biochemistry

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