Abstract
Apolipoprotein A-IV (apo A-IV) is present in plasma associated to both HDL and as a complex with lipids that cannot be flotated by ultracentrifugation at 1.21 g/ml density. Apo A-IV is likely an important molecular determinant in HDL binding to the liver. In this communication, data are presented supporting the view that a specific liver plasma membrane protein of Mr 95000 is a constituent of the apo A-IV binding site. The protein was solubilized with CHAPS from purified rat liver plasma membranes and subjected to SDS-PAGE. Transblotted to nitrocellulose sheet could be identified as recognizing 125I-apo A-IV-DMPC by autoradiography. 125I-apo A-I-DMPC and radioiodinated rat apo E-poor HDL, also bound to the protein. Apo B-100 (as human LDL) and apo C-III did not bind. The protein identified is likely to be the same that has been previously identified by Graham and Oram ((1987) J. Biol. Chem. 262, 7439-7442) as 'HDL receptor protein'.
Original language | English (US) |
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Pages (from-to) | 173-175 |
Number of pages | 3 |
Journal | Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism |
Volume | 1044 |
Issue number | 1 |
DOIs | |
State | Published - May 1 1990 |
Keywords
- (Rat liver)
- Apolipoprotein A-IV
- Cholesterol
- Lipid metabolism
- Membrane protein
- Receptor
- Reverse cholesterol transport
ASJC Scopus subject areas
- Endocrinology
- Biophysics
- Biochemistry