Identification of a detection panel for post-transplant virus infection through integrated analysis of non-coding RNAs in peripheral blood

Huadi Chen, Anli Yang, Chenglin Wu, Jianwei Lin, Xiaoping Wang, Mengran Peng, Dian Li, Tao Zhang, Qiang Zhao, Xiaoshun He

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Viral infection seriously affects the survival and life quality of transplanted patients without an accurate diagnosis during the early stage. Herein, we aimed to develop a novel diagnostic method based on non-coding RNAs expression in peripheral blood. An immunosuppressive mouse model of viral infection after transplantation was established. Differentially expressed non-coding RNAs were distinguished by microarray analyses in the virus-infected group. After homology analysis, 46 miRNAs and 24 lncRNAs were further verified by qRT-PCR in the peripheral blood samples of transplanted patients. Compared with normal transplanted patients, miR-29b, miR-185, and NR_073415.2 were significantly downregulated in the PBMC of post-transplant patients with viral infection. Based on the expression of the above three RNAs, principal component analysis (PCA) identified a slight overlap between the two groups. A 3-non-coding-RNA detection panel was constructed by the support vector machine analysis (SVM), whose loss rate was 14.71%. The area under the curve of it was 0.909. With the optimal cut-off value (Y = 0.328), the sensitivity was 0.929 and the specificity was 0.781. Therefore, based on non-coding RNAs expressions, a detection panel for viral infection after organ transplantation was formed with high diagnostic specificity and sensitivity.

Original languageEnglish (US)
Pages (from-to)691-698
Number of pages8
JournalArtificial Cells, Nanomedicine and Biotechnology
Volume49
Issue number1
DOIs
StatePublished - 2021

Keywords

  • NR_073415.2
  • Viral infection
  • diagnosis
  • miR-185
  • miR-29b
  • non-coding RNA
  • transplantation

ASJC Scopus subject areas

  • Biotechnology
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Pharmaceutical Science

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