TY - JOUR
T1 - Identification, cloning, and sequencing of DNA essential for encapsulation of Streptococcus pneumoniae
AU - Watson, David A.
AU - Kapur, Vivek
AU - Musher, Daniel M.
AU - Jacobson, James W.
AU - Musser, James M.
PY - 1995/10
Y1 - 1995/10
N2 - This paper reports the cloning and sequencing of a region of DNA from Streptococcus pneumoniae serotype 3 surrounding transposon Tn 916, insertion of which was previously shown to result in lack of expression of the extracellular capsule. Sequence analysis revealed that the transposon inserted into a consensus insertion site 71 bp from the 5′ end of the cloned fragment. Within the clone, 3′ downstream regions from two different pneumococcal lytA genes were identified, as well as a putative 194 AA open reading frame (ORF1). Moreover, two copies of the repeat element BOX, oriented in opposite directions, were located immediately 3′ of orf1. Within the region bounded by the first pair of internal sequencing primers, analysis revealed that the fragment amplified by PCR was always of the same size. Moreover, Southern blotting showed that for all serotypes examined to date, homology exists with the cloned fragment. These results indicate that this region of the chromosome is highly conserved and, taken together with other independently derived data, suggest that interruptions or deletions within this DNA lead to unencapsulation.
AB - This paper reports the cloning and sequencing of a region of DNA from Streptococcus pneumoniae serotype 3 surrounding transposon Tn 916, insertion of which was previously shown to result in lack of expression of the extracellular capsule. Sequence analysis revealed that the transposon inserted into a consensus insertion site 71 bp from the 5′ end of the cloned fragment. Within the clone, 3′ downstream regions from two different pneumococcal lytA genes were identified, as well as a putative 194 AA open reading frame (ORF1). Moreover, two copies of the repeat element BOX, oriented in opposite directions, were located immediately 3′ of orf1. Within the region bounded by the first pair of internal sequencing primers, analysis revealed that the fragment amplified by PCR was always of the same size. Moreover, Southern blotting showed that for all serotypes examined to date, homology exists with the cloned fragment. These results indicate that this region of the chromosome is highly conserved and, taken together with other independently derived data, suggest that interruptions or deletions within this DNA lead to unencapsulation.
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U2 - 10.1007/BF00298383
DO - 10.1007/BF00298383
M3 - Article
C2 - 7549771
AN - SCOPUS:0029091603
SN - 0343-8651
VL - 31
SP - 251
EP - 259
JO - Current Microbiology
JF - Current Microbiology
IS - 4
ER -