Identification and characterization of a novel heme-associated cell surface protein made by Streptococcus pyogenes

Benfang Lei, Laura M. Smoot, Heather M. Menning, Jovanka M. Voyich, Subbarao V. Kala, Frank R. Deleo, Sean D. Reid, James M. Musser

Research output: Contribution to journalArticlepeer-review

90 Scopus citations


Analysis of the genome sequence of a serotype M1 group A Streptococcus (GAS) strain identified a gene encoding a previously undescribed putative cell surface protein. The gene was cloned from a serotype M1 strain, and the recombinant protein was overexpressed in Escherichia coli and purified to homogeneity. The purified protein was associated with heme in a 1:1 stoichiometry. This streptococcal heme-associated protein, designated Shp, was produced in vitro by GAS, located on the bacterial cell surface, and accessible to specific antibody raised against the purified recombinant protein. Mice inoculated subcutaneously with GAS and humans with invasive infections and pharyngitis caused by GAS seroconverted to Shp, indicating that Shp was produced in vivo. The blood of mice actively immunized with Shp had significantly higher bactericidal activity than the blood of unimmunized mice. The shp gene was cotranscribed with eight contiguous genes, including homologues of an ABC transporter involved in iron uptake in gram-negative bacteria. Our results indicate that Shp is a novel cell surface heme-associated protein.

Original languageEnglish (US)
Pages (from-to)4494-4500
Number of pages7
JournalInfection and Immunity
Issue number8
StatePublished - 2002

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases


Dive into the research topics of 'Identification and characterization of a novel heme-associated cell surface protein made by Streptococcus pyogenes'. Together they form a unique fingerprint.

Cite this