ID1 is a functional marker for intestinal stem and progenitor cells required for normal response to injury

Ning Zhang, Rhonda K. Yantiss, Hyung Song Nam, Yvette Chin, Xi Kathy Zhou, Ellen J. Scherl, Brian P. Bosworth, Kotha Subbaramaiah, Andrew J. Dannenberg, Robert Benezra

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

LGR5 and BMI1 mark intestinal stem cells in crypt base columnar cells and +4 position cells, respectively, but characterization of functional markers in these cell populations is limited. ID1 maintains the stem cell potential of embryonic, neural, and longterm repopulating hematopoietic stem cells. Here, we show in both human and mouse intestine that ID1 is expressed in cycling columnar cells, +4 position cells, and transit-amplifying cells in the crypt. Lineage tracing revealed ID1+ cells to be self-renewing, multipotent stem/progenitor cells that are responsible for the long-term renewal of the intestinal epithelium. Single ID1+ cells can generate long-lived organoids resembling mature intestinal epithelium. Complete knockout of Id1 or selective deletion of Id1 in intestinal epithelium or in LGR5+ stem cells sensitizes mice to chemical-induced colon injury. These experiments identify ID1 as a marker for intestinal stem/progenitor cells and demonstrate a role for ID1 in maintaining the potential for repair in response to colonic injury.

Original languageEnglish (US)
Pages (from-to)716-724
Number of pages9
JournalStem Cell Reports
Volume3
Issue number5
DOIs
StatePublished - 2014

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

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