Abstract
In the post-genomics era, proteomics has become a central branch in life sciences. An understanding of biological functions will not only rely on protein identification, but also on protein quantification in a living organism. Most of the existing methods for quantitative proteomics are based on isotope labeling combined with molecular mass spectrometry. Recently, a remarkable progress that utilizes inductively coupled plasmamass spectrometry (ICP-MS) as an attractive complement to electrospray MS and MALDI MS for protein quantification, especially for absolute quantification, has been achieved. This review will selectively discuss the recent advances of ICP-MSbased technique, which will be expected to further mature and to become one of the key methods in quantitative proteomics.
Original language | English (US) |
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Pages (from-to) | 326-348 |
Number of pages | 23 |
Journal | Mass Spectrometry Reviews |
Volume | 29 |
Issue number | 2 |
DOIs | |
State | Published - Mar 2010 |
Keywords
- Elemental labeling
- Elemental mass spectrometry
- Isotope dilution
- Laser ablation
- Molecular mass spectrometry
- Quantitative proteomics
ASJC Scopus subject areas
- Condensed Matter Physics
- Analytical Chemistry
- Spectroscopy
- Biochemistry, Genetics and Molecular Biology(all)