Icp-ms-based strategies for protein quantification

Meng Wang, Wei Yue Feng, Yu Liang Zhao, Zhi Fang Chai

Research output: Contribution to journalArticle

91 Scopus citations

Abstract

In the post-genomics era, proteomics has become a central branch in life sciences. An understanding of biological functions will not only rely on protein identification, but also on protein quantification in a living organism. Most of the existing methods for quantitative proteomics are based on isotope labeling combined with molecular mass spectrometry. Recently, a remarkable progress that utilizes inductively coupled plasmamass spectrometry (ICP-MS) as an attractive complement to electrospray MS and MALDI MS for protein quantification, especially for absolute quantification, has been achieved. This review will selectively discuss the recent advances of ICP-MSbased technique, which will be expected to further mature and to become one of the key methods in quantitative proteomics.

Original languageEnglish (US)
Pages (from-to)326-348
Number of pages23
JournalMass Spectrometry Reviews
Volume29
Issue number2
DOIs
StatePublished - Mar 1 2010

Keywords

  • Elemental labeling
  • Elemental mass spectrometry
  • Isotope dilution
  • Laser ablation
  • Molecular mass spectrometry
  • Quantitative proteomics

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Analytical Chemistry
  • Spectroscopy
  • Biochemistry, Genetics and Molecular Biology(all)

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