Hyperglycemia Accentuates Persistent "Functional Uncoupling" of Cerebral Microvascular Nitric Oxide and Superoxide Following Focal Ischemia/Reperfusion in Rats

Roderic H. Fabian, Thomas A. Kent

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


Hyperglycemia worsens outcome in stroke patients and in a variety of stroke models, most prominently following prolonged ischemia and reperfusion. Vascular dysfunction has been associated with this worsened outcome, manifested by reduced reperfusion cerebral blood flow (CBF), as well as increased hemorrhagic transformation, edema, and mortality. The phenomenon of "uncoupling" of endothelial nitric oxide synthase (eNOS) has been associated with hyperglycemia in the periphery and results in a dysfunctional eNOS-generating superoxide radical (O2-) in lieu of nitric oxide (NO). A net result of NOS uncoupling is reduction in blood flow and generation of a pro-thrombotic, inflammatory vascular phenotype that could explain many of the features of hyperglycemic stroke. Because the sources of increased O2- and mechanisms of reduced NO are difficult to identify under in vivo pathological conditions, we have termed the presence of perivascular excess of O2- expression relative to NO as "functional uncoupling." We hypothesized that hyperglycemia would induce a state of functional uncoupling in the cerebral microvasculature that would be exacerbated by transient focal ischemia. We examined the relative expression of systemically injected radical sensitive dyes in cerebral microvessel profiles in the basal and 24 h post-stroke brain in a rat model of middle cerebral artery occlusion and recanalization (MCAO/R) in controls and after hyperglycemia. We focused on the peri-infarct region because of its importance in penumbra extension and edema. Differential expression of fluorescent dyes sensitive to O2- and NO in microvessel profiles were assessed in the peri-infarct region. Hyperglycemia was induced by streptozotocin 48 h prior to MCAO/R. We found that hyperglycemia resulted in an increase in O2- relative to NO, a pattern consistent with functional uncoupling. This ratio was accentuated 24 h after MCA/O in both groups. Hyperglycemic rats showed a synergistic increase in the O2-/NO ratio as well as reduced acute reperfusion CBF, increased infarct size, and enhanced expression of nitrotyrosine. While effects of hyperglycemia on oxidative radicals is well known, we showed for the first time that hyperglycemia synergistically worsened functional uncoupling in the peri-infarct microvasculature and that it persisted for the 24-h duration of the experiment. Persistent generation of microvascular O2- and reduction in NO expression suggest potential late therapeutic targets to restore microvessel function and improve vascular outcomes in hyperglycemic stroke.

Original languageEnglish (US)
Pages (from-to)482-490
Number of pages9
JournalTranslational Stroke Research
Issue number4
StatePublished - Dec 2012


  • Cerebral blood flow
  • Cerebral infarction
  • Hyperglycemia
  • Nitric oxide
  • Superoxide anion

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine


Dive into the research topics of 'Hyperglycemia Accentuates Persistent "Functional Uncoupling" of Cerebral Microvascular Nitric Oxide and Superoxide Following Focal Ischemia/Reperfusion in Rats'. Together they form a unique fingerprint.

Cite this