TY - JOUR
T1 - Hydroxyapatite nanocrystals functionalized with alendronate as bioactive components for bone implant coatings to decrease osteoclastic activity
AU - Bosco, Ruggero
AU - Iafisco, Michele
AU - Tampieri, Anna
AU - Jansen, John A.
AU - Leeuwenburgh, Sander C.G.
AU - Van Den Beucken, Jeroen J.J.P.
PY - 2015/2/15
Y1 - 2015/2/15
N2 - The integration of bone implants within native bone tissue depends on periprosthetic bone quality, which is severely decreased in osteoporotic patients. In this work, we have synthesized bone-like hydroxyapatite nanocrystals (nHA) using an acid-base neutralization reaction and analysed their physicochemical properties. Subsequently, we have functionalized the nHA with alendronate (nHA ALE ), a well-known bisphosphonate drug used for the treatment of osteoporosis. An in vitro osteoclastogenesis test was carried out to evaluate the effect of nHA ALE on the formation of osteoclast-like cells from monocytic precursor cells (i.e. RAW264.7 cell line) showing that nHA ALE significantly promoted apoptosis of osteoclast-like cells. Subsequently, nHA and nHA ALE were deposited on titanium disks using electrospray deposition (ESD), for which characterisation of the deposited coatings confirmed the presence of alendronate in nHA ALE coatings with nanoscale thickness of about 700 nm. These results indicate that alendronate linked to hydroxyapatite nanocrystals has therapeutic potential and nHA ALE can be considered as an appealing coating constituent material for orthopaedic and oral implants for application in osteoporotic patients.
AB - The integration of bone implants within native bone tissue depends on periprosthetic bone quality, which is severely decreased in osteoporotic patients. In this work, we have synthesized bone-like hydroxyapatite nanocrystals (nHA) using an acid-base neutralization reaction and analysed their physicochemical properties. Subsequently, we have functionalized the nHA with alendronate (nHA ALE ), a well-known bisphosphonate drug used for the treatment of osteoporosis. An in vitro osteoclastogenesis test was carried out to evaluate the effect of nHA ALE on the formation of osteoclast-like cells from monocytic precursor cells (i.e. RAW264.7 cell line) showing that nHA ALE significantly promoted apoptosis of osteoclast-like cells. Subsequently, nHA and nHA ALE were deposited on titanium disks using electrospray deposition (ESD), for which characterisation of the deposited coatings confirmed the presence of alendronate in nHA ALE coatings with nanoscale thickness of about 700 nm. These results indicate that alendronate linked to hydroxyapatite nanocrystals has therapeutic potential and nHA ALE can be considered as an appealing coating constituent material for orthopaedic and oral implants for application in osteoporotic patients.
KW - Bisphosphonate
KW - Coating
KW - Electrospray
KW - Hydroxyapatite
KW - Osteoclast
KW - Osteoporosis
UR - http://www.scopus.com/inward/record.url?scp=84922229216&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84922229216&partnerID=8YFLogxK
U2 - 10.1016/j.apsusc.2014.12.072
DO - 10.1016/j.apsusc.2014.12.072
M3 - Article
AN - SCOPUS:84922229216
VL - 328
SP - 516
EP - 524
JO - Applied Surface Science
JF - Applied Surface Science
SN - 0169-4332
ER -