Human TNF receptor-associated factor 5 (TRAF5): cDNA cloning, expression and assignment of the TRAF5 gene to chromosome 1q32

Hiroyasu Nakano, Masahisa Shindo, Kazuhiko Yamada, Michihiro C. Yoshida, Sybil M. Santee, Carl F. Ware, Nancy A. Jenkins, Debra J. Gilbert, Hideo Yagita, Neal G. Copeland, Ko Okumura

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Tumor necrosis factor (TNF) receptor-associated factors (TRAFs) are signal transducers for members of the TNF receptor superfamily. We previously identified murine TRAF5 (mTRAF5) and showed that it specifically interacts with the lymphotoxin-β receptor (LT-βR) and activates the transcription factor NF-κB. Here we have cloned the human TRAF5 homologue (hTRAF5) by cross hybridization with mTRAF5 cDNA. hTRAF5 cDNA is composed of 2894 nucleotides with a 557-amino-acid open reading frame that exhibits 77.5 and 80% identity to mTRAF5 at the nucleotide and amino acid levels, respectively. Northern blot analysis revealed that hTRAF5 mRNA is expressed in all visceral organs. Western blotting revealed that hTRAF5 protein was abundantly expressed in the human follicular dentritic cell line, FDC1, and to a much lesser degree in several tumor cell lines. Interspecific backcross mapping revealed that Traf5 is located in the distal region of mouse chromosome 1, which shares a region of homology with human chromosome 1q. Fluorescence in situ hybridization confirmed regional localization to human chromosome 1q32.

Original languageEnglish (US)
Pages (from-to)26-32
Number of pages7
JournalGenomics
Volume42
Issue number1
DOIs
StatePublished - May 15 1997

ASJC Scopus subject areas

  • Genetics

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