TY - JOUR
T1 - Human large granular lymphocytes induce immunoglobulin synthesis after bone marrow transplantation
AU - Brenner, Malcolm K.
AU - Vyakarnam, Anna
AU - Reittie, Joyce E.
AU - Wimperis, Jennifer Z.
AU - Grob, Jean Philippe
AU - Hoffbrand, A. Victor
AU - Prentice, H. Grant
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1987
Y1 - 1987
N2 - Following T cell-depleted bone marrow transplantation, helper T cell numbers remain depressed for some months. Nonetheless, functional B cells can be adoptively transferred to the recipients of such grafts, where they continue to secrete antibody. We now show that immunoglobulin production by these transferred B cells is induced by activated large granular lymphocytes (LGL) which circulate in the recipients in substantial numbers during the immediate post-transplant period. The LGL are CD3 negative and therefore provide help in an antigen-unlinked manner. Helper effects for autologous (donor) B cells are augmented by the addition of anti-LFA-2 (anti-CD2) which appears to act by blocking recruitment of LGL inhibitory to developing B cells. In contrast antibody to the β chain of LFA-1, which effectively reduces natural killer activity of LGL, does not influence their helper function. The peripheral blood LGL fraction thus contains both helper and cytotoxic activity, which can be distinguished by appropriate monoclonal antibodies.
AB - Following T cell-depleted bone marrow transplantation, helper T cell numbers remain depressed for some months. Nonetheless, functional B cells can be adoptively transferred to the recipients of such grafts, where they continue to secrete antibody. We now show that immunoglobulin production by these transferred B cells is induced by activated large granular lymphocytes (LGL) which circulate in the recipients in substantial numbers during the immediate post-transplant period. The LGL are CD3 negative and therefore provide help in an antigen-unlinked manner. Helper effects for autologous (donor) B cells are augmented by the addition of anti-LFA-2 (anti-CD2) which appears to act by blocking recruitment of LGL inhibitory to developing B cells. In contrast antibody to the β chain of LFA-1, which effectively reduces natural killer activity of LGL, does not influence their helper function. The peripheral blood LGL fraction thus contains both helper and cytotoxic activity, which can be distinguished by appropriate monoclonal antibodies.
UR - http://www.scopus.com/inward/record.url?scp=0023156947&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023156947&partnerID=8YFLogxK
U2 - 10.1002/eji.1830170108
DO - 10.1002/eji.1830170108
M3 - Article
C2 - 3545853
AN - SCOPUS:0023156947
VL - 17
SP - 43
EP - 47
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 1
ER -