Human glioma-specific antigens detected by monoclonal antibodies

Seiji Kondo, Shin Ichi Miyatake, Koh Ichi Iwasaki, Yoshifumi Oda, Haruhiko Kikuchi, Youli Zu, Mikihiro Shamoto, Yuziro Namba

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Three murine monoclonal antibodies, designated GA-17, GB-4, and GC-3, were prepared by the hybridization of murine myeloma cells (NS-1) and spleen cells of BALB/c mice immunized with the crude membrane fraction of cultured human gliosarcoma cells (GI-1). Two of them (GA-17 and GB-4) reacted exclusively with the membrane of glioma cells, and the other (GC-3) reacted with the membrane of glioma cells and a T cell line (MOLT-4). Although these antibodies reacted with almost all of the gliomas, the reactions differed. GA-17 reacted equally well with all glioblastoma (17 cases) and low-grade astrocytoma (10 cases), whereas GB-4 reacted poorly with 7 cases of glioblastoma and GC-3 did not react with 7 cases of low-grade astrocytoma. The antigens, exclusively expressed on the cell surface, were analyzed by surface labeling with 125I followed by a cell lysis and immunoprecipitation with these antibodies. The findings obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that GA-17, GB-4, and GC-3 reacted with Mr 140,000–145,000, Mr 160,000, and Mr 145,000–150,000 proteins, respectively. Some evidence has been obtained indicating that these antigens are composed of the same polypeptide chain (Mr 120,000) with the carbohydrate chains being different.

Original languageEnglish (US)
Pages (from-to)506-511
Number of pages6
JournalNeurosurgery
Volume30
Issue number4
DOIs
StatePublished - Apr 1992

Keywords

  • C-kit protein
  • Glioblastoma
  • Glioma-specific antigen
  • Low-grade astrocytoma
  • Monoclonal antibody

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology

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