Human DNA polymerase η promotes RNA-templated error-free repair of DNA double-strand breaks

Anirban Chakraborty, Nisha Tapryal, Azharul Islam, Altaf H. Sarker, Kodavati Manohar, Joy Mitra, Muralidhar L. Hegde, Tapas Hazra

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


A growing body of evidence indicates that RNA plays a critical role in orchestrating DNA double-strand break repair (DSBR). Recently, we showed that homologous nascent RNA can be used as a template for error-free repair of double-strand breaks (DSBs) in the transcribed genome and to restore the missing sequence at the break site via the transcription-coupled classical nonhomologous end-joining (TC-NHEJ) pathway. TC-NHEJ is a complex multistep process in which a reverse transcriptase (RT) is essential for synthesizing the DNA strand from template RNA. However, the identity of the RT involved in the TC-NHEJ pathway remained unknown. Here, we report that DNA polymerase eta (Pol η), known to possess RT activity, plays a critical role in TC-NHEJ. We found that Pol η forms a multiprotein complex with RNAP II and other TC-NHEJ factors, while also associating with nascent RNA. Moreover, purified Pol η, along with DSBR proteins PNKP, XRCC4, and Ligase IV can fully repair RNA templated 3′-phosphate-containing gapped DNA substrate. In addition, we demonstrate here that Pol η deficiency leads to accumulation of R-loops and persistent strand breaks in the transcribed genes. Finally, we determined that, in Pol η depleted but not in control cells, TC-NHEJ-mediated repair was severely abrogated when a reporter plasmid containing a DSB with several nucleotide deletion within the E. coli lacZ gene was introduced for repair in lacZ-expressing mammalian cells. Thus, our data strongly suggest that RT activity of Pol η is required in error-free DSBR.

Original languageEnglish (US)
Article number102991
JournalJournal of Biological Chemistry
Issue number3
StatePublished - Mar 2023


  • DNA polymerase eta
  • double strand break repair
  • RNA-templated DNA polymerase activity
  • RNA-templated repair
  • transcription-coupled NHEJ
  • Humans
  • Phosphotransferases (Alcohol Group Acceptor)/genetics
  • Escherichia coli/genetics
  • Mammals
  • DNA Breaks, Double-Stranded
  • RNA/genetics
  • Animals
  • DNA Repair
  • DNA End-Joining Repair
  • DNA
  • DNA Ligase ATP
  • DNA Repair Enzymes/genetics

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry
  • Cell Biology


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