TY - JOUR
T1 - Human breast cancer cell lines as models for investigating the effects of 2,3,7,8-TCDD and related compounds
AU - Harris, M.
AU - Piskorska-Pliszczynska, J.
AU - Zacharewski, T.
AU - Safe, S.
N1 - Funding Information:
ACKNOWLEDGEMENTS The financial assistance of the National Institutes of Health (ES03554 and ES04176) and the Texas Agricultural Experiment Station is gratefulIy acknowledged.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1990
Y1 - 1990
N2 - The dose-response effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 2,3,7,8-tetrachlorodibenzofuran (TCDF) and 1,2,4,7,8-pentachlorodibenzo-p-dioxin (PeCDD) as inducers of aryl hydrocarbon hydroxylase (AHH) and ethoxyresorufin O-deethylase (EROD) were determined for 3 human breast cancer cell lines. The T47-D and MCF-7 cell lines were highly responsive to the induction effects of TCDD and TCDF. EC50 values were approximately 1 × 10-9 M for TCDD and TCDF in both cell lines and in both enzyme assays. These results, coupled with the low activity of the PeCDD congener were consistent with an Ah receptor mediated-induction process. The MDA-MB-231 cell line was not responsive to TCDD, TCDF or PeCDD. Preliminary data demonstrated that pretreatment of MCF-7 cells with TCDD resulted in a substantial decrease in the amount of nuclear estrogen receptor bound by [3H]-estradiol. Additional studies using estrogen receptor antibodies confirmed the reduction of the nuclear estrogen receptor protein in MCF-7 cells by TCDD.
AB - The dose-response effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 2,3,7,8-tetrachlorodibenzofuran (TCDF) and 1,2,4,7,8-pentachlorodibenzo-p-dioxin (PeCDD) as inducers of aryl hydrocarbon hydroxylase (AHH) and ethoxyresorufin O-deethylase (EROD) were determined for 3 human breast cancer cell lines. The T47-D and MCF-7 cell lines were highly responsive to the induction effects of TCDD and TCDF. EC50 values were approximately 1 × 10-9 M for TCDD and TCDF in both cell lines and in both enzyme assays. These results, coupled with the low activity of the PeCDD congener were consistent with an Ah receptor mediated-induction process. The MDA-MB-231 cell line was not responsive to TCDD, TCDF or PeCDD. Preliminary data demonstrated that pretreatment of MCF-7 cells with TCDD resulted in a substantial decrease in the amount of nuclear estrogen receptor bound by [3H]-estradiol. Additional studies using estrogen receptor antibodies confirmed the reduction of the nuclear estrogen receptor protein in MCF-7 cells by TCDD.
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U2 - 10.1016/0045-6535(90)90233-J
DO - 10.1016/0045-6535(90)90233-J
M3 - Article
AN - SCOPUS:0024992086
SN - 0045-6535
VL - 20
SP - 1135
EP - 1140
JO - Chemosphere
JF - Chemosphere
IS - 7-9
ER -