Human anti-tetanus antibody response in vitro: Autologous and allogeneic T cells provide help by different routes

Malcolm Brenner, A. J. Munro

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Human B cells will make anti-tetanus antibody in vitro in the presence of antigen and T cells. These T cells may be autologous, but allogeneic T lymphocytes function equally well provided they are first irradiated. The allogeneic cells provide help by a different route. Co-culture of allogeneic cells produces a degree of polyclonal activation of B cells and a much higher level of IgM anti-tetanus antibody than autologous cultures. Depletion of tetanus toxoid or alloantigen-reactive T cells by 3H-thymidine suicide indicates that in autologous cultures help for anti-tetanus toxoid antibody production is provided by antigen-reactive T cells while in allogeneic cultures antibody production is dependent on the presence of alloreactive T cells. The implications for assessing human T and B cell function are discussed.

Original languageEnglish (US)
Pages (from-to)171-177
Number of pages7
JournalClinical and Experimental Immunology
Volume46
Issue number1
StatePublished - Jan 1 1981

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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