TY - JOUR
T1 - HPV E7 viral oncoprotein disrupts transcriptional regulation of L1Md retrotransposon
AU - Montoya-Durango, Diego E.
AU - Ramos, Kenneth S.
N1 - Funding Information:
This work was supported in part by National Institutes of Health (NIH, USA). [Grants 5R01 ES004849; ARRA GB090603A1, Kentucky Lung Cancer Research Program, and P30 ES014443 to K.S.R.]
PY - 2012/1/2
Y1 - 2012/1/2
N2 - Murine L1Md-A5 retrotransposon is a redox-inducible element regulated by Nrf-2/JunD and E2F/Rb-binding sites within its promoter (5′-UTR). Because the human papillomavirus (HPV) oncoprotein E7 interacts with retinoblastoma (pRb) and members of the AP1 family, studies were conducted to examine functional interactions between HPV E7, pRb, and histone deacetylase 2 (HDAC2) in the regulation of L1Md-A5. Using a transient heterologous transcription system we found that HPV E7 alone, or in combination with HDAC2, disrupted pRb-mediated L1MdA-5 transactivation. HPV E7 also ablated the transcriptional response of L1Md-A5 to genotoxic stress, but did not interfere with basal activity. We conclude that HPV E7 associates with proteins involved in the assembly of macromolecular complexes that regulate antioxidant and E2F/Rb sites within L1MdA-5 to regulate biological activity
AB - Murine L1Md-A5 retrotransposon is a redox-inducible element regulated by Nrf-2/JunD and E2F/Rb-binding sites within its promoter (5′-UTR). Because the human papillomavirus (HPV) oncoprotein E7 interacts with retinoblastoma (pRb) and members of the AP1 family, studies were conducted to examine functional interactions between HPV E7, pRb, and histone deacetylase 2 (HDAC2) in the regulation of L1Md-A5. Using a transient heterologous transcription system we found that HPV E7 alone, or in combination with HDAC2, disrupted pRb-mediated L1MdA-5 transactivation. HPV E7 also ablated the transcriptional response of L1Md-A5 to genotoxic stress, but did not interfere with basal activity. We conclude that HPV E7 associates with proteins involved in the assembly of macromolecular complexes that regulate antioxidant and E2F/Rb sites within L1MdA-5 to regulate biological activity
KW - 5′-Untranslated region (5′-UTR)
KW - Benzo(a)pyrene (BaP)
KW - E7 viral oncoprotein
KW - Long interspersed nuclear element (LINE1 or L1)
KW - Retinoblastoma protein family
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U2 - 10.1016/j.febslet.2011.12.005
DO - 10.1016/j.febslet.2011.12.005
M3 - Article
C2 - 22172279
AN - SCOPUS:84655167540
SN - 0014-5793
VL - 586
SP - 102
EP - 106
JO - FEBS Letters
JF - FEBS Letters
IS - 1
ER -