HPLC determination of tobramycin in a simulated body fluid

Federica Valentini, Pier Luigi Buldini, Elena Landi, Anna Tampieri, Domenica Tonelli

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


To evaluate the release kinetics, in a simulated body fluid, of tobramycin (TOB) from carbonated apatite (CHA) in the form of granulate, a sensitive and reliable liquid chromatographic method was developed. Chromatographic separation was performed on a Metrosep Carb 1 anionic column by using 0.1 M NaOH as mobile phase at a flow rate of 1.0 ml min- 1. TOB was quantified by pulsed amperometric detection using a 3-potential waveform on Au electrode. A linear relationship for TOB was found in the range 0.17-2.50 mg l- 1 with a LOD of 0.05 mg l- 1. The method exhibited good retention time and peak area reproducibility.

Original languageEnglish (US)
Pages (from-to)113-117
Number of pages5
JournalMicrochemical Journal
Issue number2
StatePublished - Dec 2008


  • Carbonated-hydroxyapatite
  • HPLC
  • Release kinetics
  • Simulated body fluid
  • Tobramycin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy


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