Abstract
To evaluate the release kinetics, in a simulated body fluid, of tobramycin (TOB) from carbonated apatite (CHA) in the form of granulate, a sensitive and reliable liquid chromatographic method was developed. Chromatographic separation was performed on a Metrosep Carb 1 anionic column by using 0.1 M NaOH as mobile phase at a flow rate of 1.0 ml min- 1. TOB was quantified by pulsed amperometric detection using a 3-potential waveform on Au electrode. A linear relationship for TOB was found in the range 0.17-2.50 mg l- 1 with a LOD of 0.05 mg l- 1. The method exhibited good retention time and peak area reproducibility.
Original language | English (US) |
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Pages (from-to) | 113-117 |
Number of pages | 5 |
Journal | Microchemical Journal |
Volume | 90 |
Issue number | 2 |
DOIs | |
State | Published - Dec 2008 |
Keywords
- Carbonated-hydroxyapatite
- HPLC
- Release kinetics
- Simulated body fluid
- Tobramycin
ASJC Scopus subject areas
- Analytical Chemistry
- Spectroscopy