HPLC determination of tobramycin in a simulated body fluid

Federica Valentini; Pier Luigi Buldini; Elena Landi; Anna Tampieri; Domenica Tonelli

doi:10.1016/j.microc.2008.04.003

HPLC determination of tobramycin in a simulated body fluid

Federica Valentini, Pier Luigi Buldini, Elena Landi, Anna Tampieri, Domenica Tonelli

Houston Methodist

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

To evaluate the release kinetics, in a simulated body fluid, of tobramycin (TOB) from carbonated apatite (CHA) in the form of granulate, a sensitive and reliable liquid chromatographic method was developed. Chromatographic separation was performed on a Metrosep Carb 1 anionic column by using 0.1 M NaOH as mobile phase at a flow rate of 1.0 ml min^{- 1}. TOB was quantified by pulsed amperometric detection using a 3-potential waveform on Au electrode. A linear relationship for TOB was found in the range 0.17-2.50 mg l^{- 1} with a LOD of 0.05 mg l^{- 1}. The method exhibited good retention time and peak area reproducibility.

Original language	English (US)
Pages (from-to)	113-117
Number of pages	5
Journal	Microchemical Journal
Volume	90
Issue number	2
DOIs	https://doi.org/10.1016/j.microc.2008.04.003
State	Published - Dec 2008

Keywords

Carbonated-hydroxyapatite
HPLC
Release kinetics
Simulated body fluid
Tobramycin

ASJC Scopus subject areas

Analytical Chemistry
Spectroscopy

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10.1016/j.microc.2008.04.003

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title = "HPLC determination of tobramycin in a simulated body fluid",

abstract = "To evaluate the release kinetics, in a simulated body fluid, of tobramycin (TOB) from carbonated apatite (CHA) in the form of granulate, a sensitive and reliable liquid chromatographic method was developed. Chromatographic separation was performed on a Metrosep Carb 1 anionic column by using 0.1 M NaOH as mobile phase at a flow rate of 1.0 ml min- 1. TOB was quantified by pulsed amperometric detection using a 3-potential waveform on Au electrode. A linear relationship for TOB was found in the range 0.17-2.50 mg l- 1 with a LOD of 0.05 mg l- 1. The method exhibited good retention time and peak area reproducibility.",

keywords = "Carbonated-hydroxyapatite, HPLC, Release kinetics, Simulated body fluid, Tobramycin",

author = "Federica Valentini and Buldini, {Pier Luigi} and Elena Landi and Anna Tampieri and Domenica Tonelli",

year = "2008",

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doi = "10.1016/j.microc.2008.04.003",

language = "English (US)",

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pages = "113--117",

journal = "Microchemical Journal",

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T1 - HPLC determination of tobramycin in a simulated body fluid

AU - Valentini, Federica

AU - Buldini, Pier Luigi

AU - Landi, Elena

AU - Tampieri, Anna

AU - Tonelli, Domenica

PY - 2008/12

Y1 - 2008/12

N2 - To evaluate the release kinetics, in a simulated body fluid, of tobramycin (TOB) from carbonated apatite (CHA) in the form of granulate, a sensitive and reliable liquid chromatographic method was developed. Chromatographic separation was performed on a Metrosep Carb 1 anionic column by using 0.1 M NaOH as mobile phase at a flow rate of 1.0 ml min- 1. TOB was quantified by pulsed amperometric detection using a 3-potential waveform on Au electrode. A linear relationship for TOB was found in the range 0.17-2.50 mg l- 1 with a LOD of 0.05 mg l- 1. The method exhibited good retention time and peak area reproducibility.

AB - To evaluate the release kinetics, in a simulated body fluid, of tobramycin (TOB) from carbonated apatite (CHA) in the form of granulate, a sensitive and reliable liquid chromatographic method was developed. Chromatographic separation was performed on a Metrosep Carb 1 anionic column by using 0.1 M NaOH as mobile phase at a flow rate of 1.0 ml min- 1. TOB was quantified by pulsed amperometric detection using a 3-potential waveform on Au electrode. A linear relationship for TOB was found in the range 0.17-2.50 mg l- 1 with a LOD of 0.05 mg l- 1. The method exhibited good retention time and peak area reproducibility.

KW - Carbonated-hydroxyapatite

KW - HPLC

KW - Release kinetics

KW - Simulated body fluid

KW - Tobramycin

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JO - Microchemical Journal

JF - Microchemical Journal

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ER -

HPLC determination of tobramycin in a simulated body fluid

Abstract

Keywords

ASJC Scopus subject areas

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