HIV detection by in situ hybridization based on confocal reflected light microscopy

L. C. Smith, Z. Jericevic, R. Cuellar, S. Paddock, Dorothy E. Lewis

Research output: Contribution to journalConference articlepeer-review

1 Scopus citations

Abstract

Elucidation of the pathogenesis of AIDS is confounded by the finding that few actively infected CD4+ cells (1 in 104-105) can be detected in the peripheral blood, even though there is dramatic depletion (often > 90%) of CD4+ cells as the disease progresses. A sensitive, 35S-based human immunodeficiency virus (HIV) mRNA in situ hybridization technique was coupled with a new detection method, confocal laser scanning microscopy, to examine transcriptionally active HIV-infected cells from individuals at different disease stages. An algorithm for image segmentation and analysis has been developed to determine the proportion of HIV-positive cells. Data obtained using this improved detection method suggest that there are more HIV mRNA-producing cells in HIV-infected individuals than previously thought, based on other detection methods.

Original languageEnglish (US)
Pages (from-to)224-232
Number of pages9
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume1428
StatePublished - Jan 1 1991
EventProceedings of Three-Dimensional Bioimaging Systems and Lasers in the Neurosciences - Los Angeles, CA, USA
Duration: Jan 23 1991Jan 24 1991

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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