HIV-1 replication in CD4+ T cells exploits the down-regulation of antiviral NEAT1 long non-coding RNAs following T cell activation

Hongbing Liu, Pei Wen Hu, Jacob Couturier, Dorothy E. Lewis, Andrew P. Rice

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The related NEAT1_1 and NEAT1_2 long noncoding RNAs (lnc RNAs) have been recently implicated in innate immunity against viral infection. We used CRISPR-Cas9 to generate Jurkat CD4+ T cell lines with a knockout (KO) of the NEAT1 gene. Viabilities of NEAT1 KO Jurkat lines were indistinguishable from parental Jurkat cells, as was the induction of CD69 after T cell activation. The KO lines were however more sensitive to the induction of apoptosis than parental Jurkat cells. HIV-1 replication was higher in the KO lines than parental Jurkat cells, demonstrating an anti-HIV function of NEAT1 lncRNAs. We observed a strong down-regulation of NEAT1 lncRNAs following activation of resting peripheral blood mononuclear cells and purified CD4+ T cells. These findings indicate that HIV-1 infection exploits the normal down-regulation of anti-viral NEAT1 lncRNAs in activated CD4+ T cells to enhance viral replication.

Original languageEnglish (US)
Pages (from-to)193-198
Number of pages6
JournalVirology
Volume522
DOIs
StatePublished - Sep 2018

Keywords

  • HIV
  • Long non-coding RNA
  • NEAT1 lncRNA
  • Viral replication

ASJC Scopus subject areas

  • Virology

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