Histone acetyltransferase encoded by NGG1 is required for morphological conversion and virulence of Candida albicans

De Dong Li; Beth Burgwyn Fuchs; Yan Wang; Xiao Wen Huang; Dan Dan Hu; Yan Sun; Dong Chai; Yuan Ying Jiang; Eleftherios Mylonakis

doi:10.2217/fmb-2017-0084

Histone acetyltransferase encoded by NGG1 is required for morphological conversion and virulence of Candida albicans

De Dong Li, Beth Burgwyn Fuchs, Yan Wang, Xiao Wen Huang, Dan Dan Hu, Yan Sun, Dong Chai, Yuan Ying Jiang, Eleftherios Mylonakis

Houston Methodist

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Aim: To assess the function of Ngg1 in Candida albicans and reveal the role of NGG1 in the morphological conversion and virulence of C. Albicans. Materials & methods: C. Albicans NGG1 gene was deleted in the wild-type strain SC5314 and the function of Ngg1 was assessed by western blot analysis. The phenotypes and the virulence of the ngg1 mutants were examined. Microarray analysis was performed to explore the mechanism. Results: The ngg1 mutants attenuated acetylated histone H3, obviously reduced filamentous growth and showed significantly diminished pathogenicity in all the infection models. Conclusion: This study suggested the histone acetyltransferase activity of C. Albicans Ngg1 and revealed the important role of NGG1 in morphological conversion and virulence of C. Albicans. </inline-graphic.

Original language	English (US)
Pages (from-to)	1497-1510
Number of pages	14
Journal	Future Microbiology
Volume	12
Issue number	16
DOIs	https://doi.org/10.2217/fmb-2017-0084
State	Published - Dec 2017

Keywords

Caenorhabditis elegans
Candida albicans
Galleria mellonella
histone acetyltransferase
morphological conversion
NGG1
virulence

ASJC Scopus subject areas

Microbiology
Microbiology (medical)

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title = "Histone acetyltransferase encoded by NGG1 is required for morphological conversion and virulence of Candida albicans",

abstract = "Aim: To assess the function of Ngg1 in Candida albicans and reveal the role of NGG1 in the morphological conversion and virulence of C. Albicans. Materials & methods: C. Albicans NGG1 gene was deleted in the wild-type strain SC5314 and the function of Ngg1 was assessed by western blot analysis. The phenotypes and the virulence of the ngg1 mutants were examined. Microarray analysis was performed to explore the mechanism. Results: The ngg1 mutants attenuated acetylated histone H3, obviously reduced filamentous growth and showed significantly diminished pathogenicity in all the infection models. Conclusion: This study suggested the histone acetyltransferase activity of C. Albicans Ngg1 and revealed the important role of NGG1 in morphological conversion and virulence of C. Albicans. ",

keywords = "Caenorhabditis elegans, Candida albicans, Galleria mellonella, histone acetyltransferase, morphological conversion, NGG1, virulence",

author = "Li, {De Dong} and Fuchs, {Beth Burgwyn} and Yan Wang and Huang, {Xiao Wen} and Hu, {Dan Dan} and Yan Sun and Dong Chai and Jiang, {Yuan Ying} and Eleftherios Mylonakis",

note = "Funding Information: This work was funded by the National Natural Science Foundation of China 81501730 to DD Li, 81573724 to Y Sun. All of the transcription data were deposited at the NCBI Gene Expression Omnibus (GEO) database with accession number GSE93200. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript. Publisher Copyright: {\textcopyright} 2017 2017 Future Medicine Ltd.",

year = "2017",

month = dec,

doi = "10.2217/fmb-2017-0084",

language = "English (US)",

volume = "12",

pages = "1497--1510",

journal = "Future Microbiology",

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TY - JOUR

T1 - Histone acetyltransferase encoded by NGG1 is required for morphological conversion and virulence of Candida albicans

AU - Li, De Dong

AU - Fuchs, Beth Burgwyn

AU - Wang, Yan

AU - Huang, Xiao Wen

AU - Hu, Dan Dan

AU - Sun, Yan

AU - Chai, Dong

AU - Jiang, Yuan Ying

AU - Mylonakis, Eleftherios

N1 - Funding Information: This work was funded by the National Natural Science Foundation of China 81501730 to DD Li, 81573724 to Y Sun. All of the transcription data were deposited at the NCBI Gene Expression Omnibus (GEO) database with accession number GSE93200. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript. Publisher Copyright: © 2017 2017 Future Medicine Ltd.

PY - 2017/12

Y1 - 2017/12

N2 - Aim: To assess the function of Ngg1 in Candida albicans and reveal the role of NGG1 in the morphological conversion and virulence of C. Albicans. Materials & methods: C. Albicans NGG1 gene was deleted in the wild-type strain SC5314 and the function of Ngg1 was assessed by western blot analysis. The phenotypes and the virulence of the ngg1 mutants were examined. Microarray analysis was performed to explore the mechanism. Results: The ngg1 mutants attenuated acetylated histone H3, obviously reduced filamentous growth and showed significantly diminished pathogenicity in all the infection models. Conclusion: This study suggested the histone acetyltransferase activity of C. Albicans Ngg1 and revealed the important role of NGG1 in morphological conversion and virulence of C. Albicans.

AB - Aim: To assess the function of Ngg1 in Candida albicans and reveal the role of NGG1 in the morphological conversion and virulence of C. Albicans. Materials & methods: C. Albicans NGG1 gene was deleted in the wild-type strain SC5314 and the function of Ngg1 was assessed by western blot analysis. The phenotypes and the virulence of the ngg1 mutants were examined. Microarray analysis was performed to explore the mechanism. Results: The ngg1 mutants attenuated acetylated histone H3, obviously reduced filamentous growth and showed significantly diminished pathogenicity in all the infection models. Conclusion: This study suggested the histone acetyltransferase activity of C. Albicans Ngg1 and revealed the important role of NGG1 in morphological conversion and virulence of C. Albicans.

KW - Caenorhabditis elegans

KW - Candida albicans

KW - Galleria mellonella

KW - histone acetyltransferase

KW - morphological conversion

KW - NGG1

KW - virulence

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Histone acetyltransferase encoded by NGG1 is required for morphological conversion and virulence of Candida albicans

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ASJC Scopus subject areas

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