Highly sensitive simultaneous detection of lead(II) and barium(II) with G-quadruplex DNA in α-hemolysin nanopore

Chun Yang, Lei Liu, Tao Zeng, Daowu Yang, Zhiyi Yao, Yuliang Zhao, Hai Chen Wu

Research output: Contribution to journalArticle

88 Scopus citations

Abstract

Both Pb2+ and Ba2+ can bind with high affinity to some specific DNA sequences, inducing the formation of unimolecular G-quadruplex structures. Translocation of a DNA probe containing such sequences through an α-hemolysin nanopore in the presence of Pb2+ or Ba2+ would result in much prolonged DNA translocation events. Quantification of these events can reveal the concentrations of Pb2+ or Ba2+ at as low as 0.8 nM. Besides, Pb2+ and Ba2+ in the solution can be simultaneously detected and individually identified. Furthermore, the probe is highly selective for Pb2+ and Ba 2+ detection without interference from other metal ions. This sensing strategy can be extended to many other analytes which can bind to DNA molecules with high affinity.

Original languageEnglish (US)
Pages (from-to)7302-7307
Number of pages6
JournalAnalytical Chemistry
Volume85
Issue number15
DOIs
StatePublished - Aug 6 2013

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Highly sensitive simultaneous detection of lead(II) and barium(II) with G-quadruplex DNA in α-hemolysin nanopore'. Together they form a unique fingerprint.

Cite this