Highly sensitive and selective DNA-based detection of mercury(II) with α-hemolysin nanopore

Shuang Wen, Tao Zeng, Lei Liu, Kai Zhao, Yuliang Zhao, Xianjin Liu, Hai Chen Wu

Research output: Contribution to journalArticlepeer-review

156 Scopus citations

Abstract

The duplex formation mediated by Hg 2+ in a properly designed ssDNA generates a stable hairpin structure, which greatly alters the translocation profile of the ssDNA through α-hemolysin nanopore. From the 2D-events contour plot, the presence of Hg 2+ can be confirmed in as little as 30 min at ∼7 nM or higher. The sensor is highly selective to Hg 2+, without interference from other metal ions. It can be fabricated from readily available materials, without the processes of synthesis, purification, probe-making, and so forth. This sensing strategy opens new possibilities for detecting many types of analytes which have specific interactions with DNA molecules.

Original languageEnglish (US)
Pages (from-to)18312-18317
Number of pages6
JournalJournal of the American Chemical Society
Volume133
Issue number45
DOIs
StatePublished - Nov 16 2011

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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