Highly restricted expression of Cre recombinase in cerebellar Purkinje cells

Xin Mei Zhang, Alam Hoi Lam Ng, Julian A. Tanner, Wu Tian Wu, Neal G. Copeland, Nancy A. Jenkins, Jian Dong Huang

Research output: Contribution to journalArticlepeer-review

81 Scopus citations


The Purkinje neuron, one of the most fascinating components of the cerebellar cortex, is involved in motor learning, motor coordination, and cognitive function. Purkinje cell protein 2 (Pcp2/L7) expression is highly restricted to Purkinje and retinal bipolar cells, where it has been exploited to enable highly specific, Cre recombinase-mediated, site-specific recombination. Previous studies showed that mice carrying a Cre transgene produced by insertion of Cre cDNA into a small 2.88-kb Pcp2 DNA fragment expressed Cre in Purkinje cells; however, some Cre activity was also observed outside the target tissues. Here, we used Red-mediated recombineering to insert Cre cDNA into a 173-kb BAC carrying the entire intact Pcp2 gene, and characterize the resultant BAC/Cre transgenic mice for Cre expression. We show that BAC/Cre transgenic mice have exclusive Cre expression in Purkinje and bipolar cells and nowhere else. These mice will facilitate Purkinje cell and retinal bipolar cell-specific genetic manipulation.

Original languageEnglish (US)
Pages (from-to)45-51
Number of pages7
Issue number1
StatePublished - Sep 2004


  • BAC
  • Cerebellum
  • Cre recombinase
  • L7
  • Pcp2
  • Purkinje cells
  • Transgenic mouse

ASJC Scopus subject areas

  • Genetics


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