@inbook{a7c063b66c6449a3a4276358f6cb2efb,
title = "High-throughput screening of substrate specificity for protein tyrosine phosphatases (PTPs) on phosphopeptide microarrays",
abstract = "Phosphatases are a family of enzymes responsible for the dephosphorylation of biomolecules. Phosphatases play essential roles in cell cycle regulation, signal transduction, and cellular communication. In recent years, one type of phosphatases, protein tyrosine phosphatases (PTPs), emerges as important therapeutic targets for complex and devastating diseases. Nevertheless, the physiological roles, substrate specificity, and downstream targets for PTPs remain largely unknown. To demonstrate how microarrays can be applied to characterizing PTPs, we describe here a phosphopeptide microarray strategy for activity-based high-throughput screening of PTPs substrate specificity. This is followed by a kinetic microarray assay and microplate assay to determine the rate constants of dephosphorylation by PTPs. This microarray strategy has been successfully applied to identifying several potent and selective substrates against different PTPs. These substrates could be used to design potent and selective PTPs inhibitors in the future.",
keywords = "High-throughput screening, Phosphopeptide microarrays, Protein tyrosine phosphatases, Substrate specificity",
author = "Liqian Gao and Lee, {Su Seong} and Jun Chen and Hongyan Sun and Yuliang Zhao and Zhifang Chai and Yi Hu",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",
year = "2016",
month = may,
day = "1",
doi = "10.1007/978-1-4939-3136-1_13",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "181--196",
booktitle = "Methods in Molecular Biology",
address = "United States",
}