High throughput analysis of drug effects on single breast cancer cells using droplet-microfluidic devices

Zhongcheng Gong, Fang Nie, Tianhua Zhang, Pushparaj Pathak, Zhiyong Wang, Kemi Cui, Hong Zhao, Stephen Wong, Long Que

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Scopus citations

Abstract

This paper reports the analysis of drug effects on single breast cancer cells using the droplet-microfluidic devices for the first time. Hundreds of droplets can be generated in less than 5 minutes. Each droplet serves as a nanoliter-volume titer plate. About 50% of the droplets contain a human breast cancer cell MDA-MB-231 and Cytochalasin D drug solution. The drug effects on each cell are monitored by analyzing the fluorescence image of each cell. Clear change of the cell shape and size has been observed after incubation, suggesting this approach is a potential viable technical platform for drug effect analysis and for high throughput drug screen and discovery.

Original languageEnglish (US)
Title of host publicationMEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest
Pages1015-1018
Number of pages4
DOIs
StatePublished - 2010
Event23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 - Hong Kong, China
Duration: Jan 24 2010Jan 28 2010

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010
CountryChina
CityHong Kong
Period1/24/101/28/10

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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