High content image sequence analysis for quantifying calcium signals inside cells with mutant presenilin-1 of familial alzheimer disease

Fuhai Li, Xiaobo Zhou, Jinmin Zhu, Jinwen Ma, Stephen T C Wong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Mutation of presenilin-1 (PS-1) gene is known to be closely related to the familial Alzheimer disease (FAD), whereas oxidative stress and de-regulation of calcium homeostasis are two important phases. To better understand the biological mechanisms between the two important phases, we present a computerized system of automated high content, time-lapse cellular image sequence analysis to quantify calcium signals inside the single cells with PS-1. The proposed system consists of three major components: cell segmentation, image displacement correction, and calcium signal extraction, denoising, and oscillation detection; some new algorithms are implemented. The validation indicates that the proposed system is effective and reliable. Two patterns of calcium signals: stable and temporal (oscillation) are found in this study. The experimental results confirm the hypothesis that h2o2 has regulatory effect on the calcium signal mediated by PS-1.

Original languageEnglish (US)
Title of host publication2007 IEEE/NIH Life Science Systems and Applications Workshop, LISA
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages31-34
Number of pages4
ISBN (Print)9781424418138
DOIs
StatePublished - Jan 1 2007
Event2007 IEEE/NIH Life Science Systems and Applications Workshop, LISA - Bethesda, MD, United States
Duration: Nov 8 2007Nov 9 2007

Other

Other2007 IEEE/NIH Life Science Systems and Applications Workshop, LISA
CountryUnited States
CityBethesda, MD
Period11/8/0711/9/07

ASJC Scopus subject areas

  • Computer Science Applications
  • Information Systems

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