Heterogeneity in the 5′ untranslated region of the rat glucocorticoid receptor mRNA

Katharine L. Gearing, William Cairns, Sam Okret, Jan-Ake Gustafsson

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

We have cloned several novel sequences upstream from the first coding exon of the rat glucocorticoid receptor (GR) mRNA using PCR. Analysis of these sequences in RNase protection assays showed that one of the cloned sequences represents the major GR 5′ non-coding exon which is expressed in all tissues studied, both at different stages of development and under different hormonal conditions. This major exon is homologous to the human GR 5′ untranslated region (UTR). Three other sequences were cloned, but could not be detected in the RNase protection assay, suggesting that they are only minor transcripts, at least under the varying conditions of GR expression studied. One of these sequences is identical to a previously described rat GR cDNA sequence, while another was shown to be contiguous with the rat genomic DNA sequence.

Original languageEnglish (US)
Pages (from-to)635-639
Number of pages5
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume46
Issue number5
DOIs
StatePublished - Jan 1 1993

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

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