HES-1 inhibits 17β-estradiol and heregulin-β1-mediated upregulation of E2F-1

Johan Hartman, Patrick Müller, James S. Foster, Jay Wimalasena, Jan Åke Gustafsson, Anders Ström

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

We have previously shown that expression of the transcription factor HES-1 is required for the growth-inhibitory effect of all-trans retinoic acid on MCF-7 cells. In this study, we have used T47D cells with tetracyclin-regulated expression of wild-type or a dominant-negative form of HES-1. Expression of HES-1 in T47D cells inhibited G1/S-phase transition and activation of Cdk2 elicited by estrogen. Estrogen treatment of T47D cells caused increased expression of E2F-1, and this expression was inhibited by cotreatment with all-trans retinoic acid. We show that the effect is mediated through HES-1, which directly downregulates E2F-1 expression through a CACGAG-site within the E2F-1 promoter. Furthermore, proliferation caused by heregulin-β1 treatment of T47D cells was inhibited by all-trans retinoic acid and this effect was mediated by HES-1. Interestingly, heregulin-β1-mediated upregulation of E2F-1 expression was directly inhibited by HES-1 through the same CACGAG-site as seen with estrogen-stimulated induction. In addition, we found that two important downstream target genes of estrogen and heregulin-β1 that are regulated through E2F-1, cyclin E and NPAT, were both regulated in a similar fashion by all-trans retinoic acid, and these effects were antagonized by dominant-negative HES-1. These findings establish that HES-1 inhibits both estrogen- and heregulin-β1-stimulated growth of breast cancer cells, and further suggest that growth inhibition induced in these cells by all-trans retinoic acid occurs via HES-1-mediated downregulation of E2F-1 expression.

Original languageEnglish (US)
Pages (from-to)8826-8833
Number of pages8
JournalOncogene
Volume23
Issue number54
DOIs
StatePublished - Nov 18 2004

Keywords

  • All-trans retinoic acid
  • Cell cycle
  • Cyclin E
  • HERP
  • NPAT
  • T47D, HES-6

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

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