Heat-induced SIRT1-mediated H4K16ac deacetylation impairs resection and SMARCAD1 recruitment to double strand breaks

Sharmistha Chakraborty, Mayank Singh, Raj K. Pandita, Vipin Singh, Calvin S.C. Lo, Fransisca Leonard, Nobuo Horikoshi, Eduardo G. Moros, Deblina Guha, Clayton R. Hunt, Eric Chau, Kazi M. Ahmed, Prayas Sethi, Vijaya Charaka, Biana Godin, Kalpana Makhijani, Harry Scherthan, Jeanette Deck, Michael Hausmann, Arjamand MushtaqMohammad Altaf, Kenneth S. Ramos, Krishna M. Bhat, Nitika Taneja, Chandrima Das, Tej K. Pandita

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Hyperthermia inhibits DNA double-strand break (DSB) repair that utilizes homologous recombination (HR) pathway by a poorly defined mechanism(s); however, the mechanisms for this inhibition remain unclear. Here we report that hyperthermia decreases H4K16 acetylation (H4K16ac), an epigenetic modification essential for genome stability and transcription. Heat-induced reduction in H4K16ac was detected in humans, Drosophila, and yeast, indicating that this is a highly conserved response. The examination of histone deacetylase recruitment to chromatin after heat-shock identified SIRT1 as the major deacetylase subsequently enriched at gene-rich regions. Heat-induced SIRT1 recruitment was antagonized by chromatin remodeler SMARCAD1 depletion and, like hyperthermia, the depletion of the SMARCAD1 or combination of the two impaired DNA end resection and increased replication stress. Altered repair protein recruitment was associated with heat-shock-induced γ-H2AX chromatin changes and DSB repair processing. These results support a novel mechanism whereby hyperthermia impacts chromatin organization owing to H4K16ac deacetylation, negatively affecting the HR-dependent DSB repair.

Original languageEnglish (US)
Article number104142
Pages (from-to)104142
Issue number4
StatePublished - Apr 15 2022


  • Biological sciences
  • Epigenetics
  • Molecular biology

ASJC Scopus subject areas

  • General


Dive into the research topics of 'Heat-induced SIRT1-mediated H4K16ac deacetylation impairs resection and SMARCAD1 recruitment to double strand breaks'. Together they form a unique fingerprint.

Cite this