Halogenated aryl hydrocarbon-induced suppression of the in vitro plaque-forming cell response to sheep red blood cells is not dependent on the Ah receptor

D. Davis, S. Safe

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

The immunosuppressive effects of the halogenated aromatic hydrocarbons (HAHs), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 2,3,4,7,8-pentachlorodibenzofuran (PeCDF), 2,3,7,8-tetrachlorodibenzofuran (TCDF), 1,2,3,7,9-PeCDF and 1,3,6,8-TCDF were investigated utilising the Mishell-Dutton model for in vitro immunization. The selected polychlorinated dibenzofuran congeners and 2,3,7,8-TCDD caused a concentration-dependent suppression of the splenic plaque-forming cell response to sheep red blood cells using cell cultures derived from C57BL/6 (Ah responsive) mice. Previous studies showed that there was up to a 14900-fold difference in the in vivo immunotoxicity of these compounds, however in the in vitro studies, their immunosuppressive potencies were comparable. In addition, these congeners also exhibited similar potencies using spleen cell cultures from DBA/2 (Ah-nonresponsive) mice. Previous research demonstrated that α-naphthoflavone was relatively inactive in the in vitro splenic assay system and that co-treatment of cells from C57BL/6 mice with α-naphthoflavone (10 μM) plus 2,3,7,8-TCDD (20 mM) resulted in a significant inhibition of the immunotoxicity of 2,3,7,8-TCDD. In these studies, comparable interactive effects were also observed in cells treated with α-naphthoflavone plus 1,3,6,8-TCDF, a weak in vivo Ah receptor agonist. Collectively, the results from this study suggest that there may be mechanism(s) of action for HAH-induced suppression of the in vitro murine humoral response to sheep red blood cells which are independent of the Ah receptor protein.

Original languageEnglish (US)
Pages (from-to)183-190
Number of pages8
JournalImmunopharmacology
Volume21
Issue number3
DOIs
StatePublished - 1991
Externally publishedYes

Keywords

  • Antagonism
  • TCDD
  • α-Naphthoflavone

ASJC Scopus subject areas

  • Pharmacology

Fingerprint Dive into the research topics of 'Halogenated aryl hydrocarbon-induced suppression of the in vitro plaque-forming cell response to sheep red blood cells is not dependent on the Ah receptor'. Together they form a unique fingerprint.

Cite this