Growth hormone regulates expression of rat liver cytochrome P-45015β at a pretranslational level

Catriona MacGeoch; Edward T. Morgan; Barbara Cordell; Jan Åke Gustafsson

doi:10.1016/0006-291X(87)91422-7

Growth hormone regulates expression of rat liver cytochrome P-450_15β at a pretranslational level

Catriona MacGeoch, Edward T. Morgan, Barbara Cordell, Jan Åke Gustafsson

Houston Methodist

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Polyclonal antibodies to rat liver microsomal cytochrome P-450_15β-hydroxylase were used to screen a female rat liver cDNA expression library. Recombinant plasmid C-6, isolated from a positive colony, contained a cDNA of 1000 base pairs which hybridized to a single, 1.7 kilobase class of mRNA on Northern blot analysis. Semi-quantitative slot blots showed that the level of this mRNA was 17-fold lower in male rat liver than in females. Continuous infusion of human growth hormone to male rats caused them to express female levels of C-6 mRNA, while the same daily dose of hormone given intermittently had no effect. A high degree of correlation of the levels of C-6 mRNA with 15β-hydroxylase protein levels and catalytic activities was found, demonstrating that the sexually differentiated, growth hormone-dependent expression of the 15β-hydroxylase is regulated at a step preceding translation.

Original language	English (US)
Pages (from-to)	782-788
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	143
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(87)91422-7
State	Published - Mar 13 1987

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(87)91422-7

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abstract = "Polyclonal antibodies to rat liver microsomal cytochrome P-45015β-hydroxylase were used to screen a female rat liver cDNA expression library. Recombinant plasmid C-6, isolated from a positive colony, contained a cDNA of 1000 base pairs which hybridized to a single, 1.7 kilobase class of mRNA on Northern blot analysis. Semi-quantitative slot blots showed that the level of this mRNA was 17-fold lower in male rat liver than in females. Continuous infusion of human growth hormone to male rats caused them to express female levels of C-6 mRNA, while the same daily dose of hormone given intermittently had no effect. A high degree of correlation of the levels of C-6 mRNA with 15β-hydroxylase protein levels and catalytic activities was found, demonstrating that the sexually differentiated, growth hormone-dependent expression of the 15β-hydroxylase is regulated at a step preceding translation.",

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AU - Cordell, Barbara

AU - Gustafsson, Jan Åke

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N2 - Polyclonal antibodies to rat liver microsomal cytochrome P-45015β-hydroxylase were used to screen a female rat liver cDNA expression library. Recombinant plasmid C-6, isolated from a positive colony, contained a cDNA of 1000 base pairs which hybridized to a single, 1.7 kilobase class of mRNA on Northern blot analysis. Semi-quantitative slot blots showed that the level of this mRNA was 17-fold lower in male rat liver than in females. Continuous infusion of human growth hormone to male rats caused them to express female levels of C-6 mRNA, while the same daily dose of hormone given intermittently had no effect. A high degree of correlation of the levels of C-6 mRNA with 15β-hydroxylase protein levels and catalytic activities was found, demonstrating that the sexually differentiated, growth hormone-dependent expression of the 15β-hydroxylase is regulated at a step preceding translation.

AB - Polyclonal antibodies to rat liver microsomal cytochrome P-45015β-hydroxylase were used to screen a female rat liver cDNA expression library. Recombinant plasmid C-6, isolated from a positive colony, contained a cDNA of 1000 base pairs which hybridized to a single, 1.7 kilobase class of mRNA on Northern blot analysis. Semi-quantitative slot blots showed that the level of this mRNA was 17-fold lower in male rat liver than in females. Continuous infusion of human growth hormone to male rats caused them to express female levels of C-6 mRNA, while the same daily dose of hormone given intermittently had no effect. A high degree of correlation of the levels of C-6 mRNA with 15β-hydroxylase protein levels and catalytic activities was found, demonstrating that the sexually differentiated, growth hormone-dependent expression of the 15β-hydroxylase is regulated at a step preceding translation.

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Growth hormone regulates expression of rat liver cytochrome P-450_15β at a pretranslational level

Abstract

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