Growth hormone regulates expression of rat liver cytochrome P-45015β at a pretranslational level

Catriona MacGeoch, Edward T. Morgan, Barbara Cordell, Jan-Ake Gustafsson

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Polyclonal antibodies to rat liver microsomal cytochrome P-45015β-hydroxylase were used to screen a female rat liver cDNA expression library. Recombinant plasmid C-6, isolated from a positive colony, contained a cDNA of 1000 base pairs which hybridized to a single, 1.7 kilobase class of mRNA on Northern blot analysis. Semi-quantitative slot blots showed that the level of this mRNA was 17-fold lower in male rat liver than in females. Continuous infusion of human growth hormone to male rats caused them to express female levels of C-6 mRNA, while the same daily dose of hormone given intermittently had no effect. A high degree of correlation of the levels of C-6 mRNA with 15β-hydroxylase protein levels and catalytic activities was found, demonstrating that the sexually differentiated, growth hormone-dependent expression of the 15β-hydroxylase is regulated at a step preceding translation.

Original languageEnglish (US)
Pages (from-to)782-788
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume143
Issue number2
DOIs
StatePublished - Mar 13 1987

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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