TY - JOUR
T1 - Growth hormone regulates expression of rat liver cytochrome P-45015β at a pretranslational level
AU - MacGeoch, Catriona
AU - Morgan, Edward T.
AU - Cordell, Barbara
AU - Gustafsson, Jan Åke
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1987/3/13
Y1 - 1987/3/13
N2 - Polyclonal antibodies to rat liver microsomal cytochrome P-45015β-hydroxylase were used to screen a female rat liver cDNA expression library. Recombinant plasmid C-6, isolated from a positive colony, contained a cDNA of 1000 base pairs which hybridized to a single, 1.7 kilobase class of mRNA on Northern blot analysis. Semi-quantitative slot blots showed that the level of this mRNA was 17-fold lower in male rat liver than in females. Continuous infusion of human growth hormone to male rats caused them to express female levels of C-6 mRNA, while the same daily dose of hormone given intermittently had no effect. A high degree of correlation of the levels of C-6 mRNA with 15β-hydroxylase protein levels and catalytic activities was found, demonstrating that the sexually differentiated, growth hormone-dependent expression of the 15β-hydroxylase is regulated at a step preceding translation.
AB - Polyclonal antibodies to rat liver microsomal cytochrome P-45015β-hydroxylase were used to screen a female rat liver cDNA expression library. Recombinant plasmid C-6, isolated from a positive colony, contained a cDNA of 1000 base pairs which hybridized to a single, 1.7 kilobase class of mRNA on Northern blot analysis. Semi-quantitative slot blots showed that the level of this mRNA was 17-fold lower in male rat liver than in females. Continuous infusion of human growth hormone to male rats caused them to express female levels of C-6 mRNA, while the same daily dose of hormone given intermittently had no effect. A high degree of correlation of the levels of C-6 mRNA with 15β-hydroxylase protein levels and catalytic activities was found, demonstrating that the sexually differentiated, growth hormone-dependent expression of the 15β-hydroxylase is regulated at a step preceding translation.
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U2 - 10.1016/0006-291X(87)91422-7
DO - 10.1016/0006-291X(87)91422-7
M3 - Article
C2 - 3494447
AN - SCOPUS:0023132003
SN - 0006-291X
VL - 143
SP - 782
EP - 788
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -