Growth factors contained in platelets enhance proliferation of human mesenchymal stem cells

Enrico Lucarelli; A. Beccheroni; A. M. Del Vento; M. Reggiani; L. Sangiorgi; L. Bonaccorsi; P. Picci; A. Cenacchi; C. Meotti; P. M. Fomasari; M. Mercuri; D. Donati

doi:10.1109/MCTE.2002.1175052

Growth factors contained in platelets enhance proliferation of human mesenchymal stem cells

Enrico Lucarelli, A. Beccheroni, A. M. Del Vento, M. Reggiani, L. Sangiorgi, L. Bonaccorsi, P. Picci, A. Cenacchi, C. Meotti, P. M. Fomasari, M. Mercuri, D. Donati

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

Mesenchymal stem cells (MSC) contained in the bone marrow provide an invaluable source for autologous progenitors cells for bone reconstruction. Major limitations in their use are: the relative low number of MSC in the marrow cell population and the small volume of bone marrow available. There are emerging data demonstrating clinical applications of ex-vivo expanded cells, whose expansion is supported by several growth factors. We investigated whether growth factors (GFs) released by platelets stimulated MSC proliferation. MTT assays showed that the effect of GFs on MSC proliferation was dose dependent and was maximal at 10%. Also frozen GFs were able to stimulate proliferation. In conclusion we identified a method to promote ex-vivo expansion of MSC to be used in bone reconstruction.

Original language	English (US)
Title of host publication	Proceedings of the IEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, MCTE 2002
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	155-156
Number of pages	2
ISBN (Electronic)	0780375572, 9780780375574
DOIs	https://doi.org/10.1109/MCTE.2002.1175052
State	Published - 2002
Event	IEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, MCTE 2002 - Genoa, Italy Duration: Jun 6 2002 → Jun 9 2002

Other

Other	IEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, MCTE 2002
Country/Territory	Italy
City	Genoa
Period	6/6/02 → 6/9/02

Keywords

Mesenchymalstem cells
Platelets
Proliferation

ASJC Scopus subject areas

Bioengineering
Medicine (miscellaneous)
Biomaterials
Biomedical Engineering
Biotechnology

Access to Document

10.1109/MCTE.2002.1175052

Cite this

Lucarelli, E., Beccheroni, A., Del Vento, A. M., Reggiani, M., Sangiorgi, L., Bonaccorsi, L., Picci, P., Cenacchi, A., Meotti, C., Fomasari, P. M., Mercuri, M., & Donati, D. (2002). Growth factors contained in platelets enhance proliferation of human mesenchymal stem cells. In Proceedings of the IEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, MCTE 2002 (pp. 155-156). [1175052] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MCTE.2002.1175052

Growth factors contained in platelets enhance proliferation of human mesenchymal stem cells. / Lucarelli, Enrico; Beccheroni, A.; Del Vento, A. M. et al.

Proceedings of the IEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, MCTE 2002. Institute of Electrical and Electronics Engineers Inc., 2002. p. 155-156 1175052.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Lucarelli, E, Beccheroni, A, Del Vento, AM, Reggiani, M, Sangiorgi, L, Bonaccorsi, L, Picci, P, Cenacchi, A, Meotti, C, Fomasari, PM, Mercuri, M & Donati, D 2002, Growth factors contained in platelets enhance proliferation of human mesenchymal stem cells. in Proceedings of the IEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, MCTE 2002., 1175052, Institute of Electrical and Electronics Engineers Inc., pp. 155-156, IEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, MCTE 2002, Genoa, Italy, 6/6/02. https://doi.org/10.1109/MCTE.2002.1175052

Lucarelli E, Beccheroni A, Del Vento AM, Reggiani M, Sangiorgi L, Bonaccorsi L et al. Growth factors contained in platelets enhance proliferation of human mesenchymal stem cells. In Proceedings of the IEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, MCTE 2002. Institute of Electrical and Electronics Engineers Inc. 2002. p. 155-156. 1175052 https://doi.org/10.1109/MCTE.2002.1175052

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abstract = "Mesenchymal stem cells (MSC) contained in the bone marrow provide an invaluable source for autologous progenitors cells for bone reconstruction. Major limitations in their use are: the relative low number of MSC in the marrow cell population and the small volume of bone marrow available. There are emerging data demonstrating clinical applications of ex-vivo expanded cells, whose expansion is supported by several growth factors. We investigated whether growth factors (GFs) released by platelets stimulated MSC proliferation. MTT assays showed that the effect of GFs on MSC proliferation was dose dependent and was maximal at 10%. Also frozen GFs were able to stimulate proliferation. In conclusion we identified a method to promote ex-vivo expansion of MSC to be used in bone reconstruction.",

keywords = "Mesenchymalstem cells, Platelets, Proliferation",

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AU - Lucarelli, Enrico

AU - Beccheroni, A.

AU - Del Vento, A. M.

AU - Reggiani, M.

AU - Sangiorgi, L.

AU - Bonaccorsi, L.

AU - Picci, P.

AU - Cenacchi, A.

AU - Meotti, C.

AU - Fomasari, P. M.

AU - Mercuri, M.

AU - Donati, D.

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AB - Mesenchymal stem cells (MSC) contained in the bone marrow provide an invaluable source for autologous progenitors cells for bone reconstruction. Major limitations in their use are: the relative low number of MSC in the marrow cell population and the small volume of bone marrow available. There are emerging data demonstrating clinical applications of ex-vivo expanded cells, whose expansion is supported by several growth factors. We investigated whether growth factors (GFs) released by platelets stimulated MSC proliferation. MTT assays showed that the effect of GFs on MSC proliferation was dose dependent and was maximal at 10%. Also frozen GFs were able to stimulate proliferation. In conclusion we identified a method to promote ex-vivo expansion of MSC to be used in bone reconstruction.

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BT - Proceedings of the IEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, MCTE 2002

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ER -

Growth factors contained in platelets enhance proliferation of human mesenchymal stem cells

Abstract

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Keywords

ASJC Scopus subject areas

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