Graph cut based active contour for automated cellular image segmentation in high throughput RNA interface (RNAi) screening

G. Chen, Houqiang Li, Xiaobo Zhou, Stephen T C Wong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

Recently, image-based, high throughput RNA interference (RNAi) experiments are increasingly carried out to facilitate the understanding of gene functions in intricate biological processes. Effective automated segmentation technique is significant in analysis of RNAi images. However, graph cuts based active contour (GCBAC) method needs interaction during segmentation. Here, we present a novel approach to overcome this shortcoming. The process consists the following steps: First, region-growing algorithm uses extracted nuclei to get the initial contours for segmentation of cytoplasm. Then, constraint factor obtained from binary segmentation of enhanced image is incorporated to improve the performance of cytoplasm segmentation. Finally, morphological thinning algorithm is implemented to solve the touching problem of clustered cells. Our approach can automatically segment clustered cells with polynomial time-consuming. The excellent results verify the effectiveness of the proposed approach.

Original languageEnglish (US)
Title of host publication2007 4th IEEE International Symposium on Biomedical Imaging
Subtitle of host publicationFrom Nano to Macro - Proceedings
Pages69-72
Number of pages4
DOIs
StatePublished - Nov 27 2007
Event2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro; ISBI'07 - Arlington, VA, United States
Duration: Apr 12 2007Apr 15 2007

Other

Other2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro; ISBI'07
CountryUnited States
CityArlington, VA
Period4/12/074/15/07

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Medicine(all)

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