Graft versus leukemia response without graft-versus-host disease elicited by adoptively transferred multivirus-specific T-cells

Jan J. Melenhorst, Paul Castillo, Patrick J. Hanley, Michael D. Keller, Robert A. Krance, Judith Margolin, Ann M. Leen, Helen E. Heslop, A. John Barrett, Cliona M. Rooney, Catherine M. Bollard

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

A 12-year-old boy with refractory acute lymphoblastic leukemia received a haploidentical transplant from his mother. As prophylaxis for Epstein-Barr virus (EBV), cytomegalovirus (CMV) and adenovirus, he received ex vivo expanded virus-specific donor T cells 3.5 months after transplant. Four weeks later leukemic blasts bearing the E2A deletion, identified by fluorescent in situ hybridization (FISH), appeared transiently in the blood followed by a FISH-negative hematological remission, which was sustained until a testicular relapse 3.5 months later. Clearance of the circulating leukemic cells coincided with a marked increase in circulating virus-specific T cells. The virus-specific cytotoxic T-cell (CTL) line showed strong polyfunctional reactivity with the patient's leukemic cells but not phytohemagglutinin (PHA) blasts, suggesting that virus-specific CTL lines may have clinically significant antileukemia activity.

Original languageEnglish (US)
Pages (from-to)179-183
Number of pages5
JournalMolecular Therapy
Volume23
Issue number1
DOIs
StatePublished - Jan 10 2015

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Pharmacology
  • Drug Discovery

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