Gold nanoparticles enhance the differentiation of embryonic stem cells into dopaminergic neurons via mTOR/p70S6K pathway

Min Wei; Song Li; Zhaofei Yang; Wei Zheng; Weidong Le

doi:10.2217/nnm-2017-0001

Gold nanoparticles enhance the differentiation of embryonic stem cells into dopaminergic neurons via mTOR/p70S6K pathway

Min Wei, Song Li, Zhaofei Yang, Wei Zheng, Weidong Le

Houston Methodist

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Aim: This study aimed to investigate the effect of gold nanoparticles (AuNPs) on differentiation of mouse embryonic stem cells (ESCs) into dopaminergic (DA) neurons and explore the possible underlying molecular mechanisms. Materials & methods: The efficiency of AuNPs on DA neuron differentiation was evaluated by observing fluorescence in TH promoter-engineered GFP-reporter ESCs, western blot and realtime PCR. The possible signal pathway was determined by western blot. Results: Compared with feeder-free control condition, AuNPs are able to enhance fate specification of ESCs into DA neurons. Moreover, mTOR/p70S6K signaling pathway was found involving in this AuNPs-mediated DA neuron differentiation. Conclusion: Our findings may lead future insight investigation into the underlying mechanisms and potential application of AuNPs in stem cell research.

Original language	English (US)
Pages (from-to)	1305-1317
Number of pages	13
Journal	Nanomedicine
Volume	12
Issue number	11
DOIs	https://doi.org/10.2217/nnm-2017-0001
State	Published - Jun 2017

Keywords

Differentiation
Dopaminergic neurons
Embryonic stem cells
Gold nanoparticles

ASJC Scopus subject areas

Bioengineering
Medicine (miscellaneous)
Biomedical Engineering
General Materials Science
Development

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10.2217/nnm-2017-0001

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title = "Gold nanoparticles enhance the differentiation of embryonic stem cells into dopaminergic neurons via mTOR/p70S6K pathway",

abstract = "Aim: This study aimed to investigate the effect of gold nanoparticles (AuNPs) on differentiation of mouse embryonic stem cells (ESCs) into dopaminergic (DA) neurons and explore the possible underlying molecular mechanisms. Materials & methods: The efficiency of AuNPs on DA neuron differentiation was evaluated by observing fluorescence in TH promoter-engineered GFP-reporter ESCs, western blot and realtime PCR. The possible signal pathway was determined by western blot. Results: Compared with feeder-free control condition, AuNPs are able to enhance fate specification of ESCs into DA neurons. Moreover, mTOR/p70S6K signaling pathway was found involving in this AuNPs-mediated DA neuron differentiation. Conclusion: Our findings may lead future insight investigation into the underlying mechanisms and potential application of AuNPs in stem cell research.",

keywords = "Differentiation, Dopaminergic neurons, Embryonic stem cells, Gold nanoparticles",

author = "Min Wei and Song Li and Zhaofei Yang and Wei Zheng and Weidong Le",

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year = "2017",

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doi = "10.2217/nnm-2017-0001",

language = "English (US)",

volume = "12",

pages = "1305--1317",

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T1 - Gold nanoparticles enhance the differentiation of embryonic stem cells into dopaminergic neurons via mTOR/p70S6K pathway

AU - Wei, Min

AU - Li, Song

AU - Yang, Zhaofei

AU - Zheng, Wei

AU - Le, Weidong

PY - 2017/6

Y1 - 2017/6

N2 - Aim: This study aimed to investigate the effect of gold nanoparticles (AuNPs) on differentiation of mouse embryonic stem cells (ESCs) into dopaminergic (DA) neurons and explore the possible underlying molecular mechanisms. Materials & methods: The efficiency of AuNPs on DA neuron differentiation was evaluated by observing fluorescence in TH promoter-engineered GFP-reporter ESCs, western blot and realtime PCR. The possible signal pathway was determined by western blot. Results: Compared with feeder-free control condition, AuNPs are able to enhance fate specification of ESCs into DA neurons. Moreover, mTOR/p70S6K signaling pathway was found involving in this AuNPs-mediated DA neuron differentiation. Conclusion: Our findings may lead future insight investigation into the underlying mechanisms and potential application of AuNPs in stem cell research.

AB - Aim: This study aimed to investigate the effect of gold nanoparticles (AuNPs) on differentiation of mouse embryonic stem cells (ESCs) into dopaminergic (DA) neurons and explore the possible underlying molecular mechanisms. Materials & methods: The efficiency of AuNPs on DA neuron differentiation was evaluated by observing fluorescence in TH promoter-engineered GFP-reporter ESCs, western blot and realtime PCR. The possible signal pathway was determined by western blot. Results: Compared with feeder-free control condition, AuNPs are able to enhance fate specification of ESCs into DA neurons. Moreover, mTOR/p70S6K signaling pathway was found involving in this AuNPs-mediated DA neuron differentiation. Conclusion: Our findings may lead future insight investigation into the underlying mechanisms and potential application of AuNPs in stem cell research.

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KW - Dopaminergic neurons

KW - Embryonic stem cells

KW - Gold nanoparticles

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Gold nanoparticles enhance the differentiation of embryonic stem cells into dopaminergic neurons via mTOR/p70S6K pathway

Abstract

Keywords

ASJC Scopus subject areas

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