Gold nanoparticles enhance the differentiation of embryonic stem cells into dopaminergic neurons via mTOR/p70S6K pathway

Min Wei, Song Li, Zhaofei Yang, Wei Zheng, Weidong Le

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Aim: This study aimed to investigate the effect of gold nanoparticles (AuNPs) on differentiation of mouse embryonic stem cells (ESCs) into dopaminergic (DA) neurons and explore the possible underlying molecular mechanisms. Materials & methods: The efficiency of AuNPs on DA neuron differentiation was evaluated by observing fluorescence in TH promoter-engineered GFP-reporter ESCs, western blot and realtime PCR. The possible signal pathway was determined by western blot. Results: Compared with feeder-free control condition, AuNPs are able to enhance fate specification of ESCs into DA neurons. Moreover, mTOR/p70S6K signaling pathway was found involving in this AuNPs-mediated DA neuron differentiation. Conclusion: Our findings may lead future insight investigation into the underlying mechanisms and potential application of AuNPs in stem cell research.

Original languageEnglish (US)
Pages (from-to)1305-1317
Number of pages13
JournalNanomedicine
Volume12
Issue number11
DOIs
StatePublished - Jun 2017

Keywords

  • Differentiation
  • Dopaminergic neurons
  • Embryonic stem cells
  • Gold nanoparticles

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)

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