TY - JOUR
T1 - Glycoconjugates in the chinchilla tubotympanum
AU - Lim, David J.
AU - Coticchia, James M.
AU - Heiselman, Fred A.
AU - Ueno, Kazuyoshi
AU - Bakaletz, Lauren O.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1991/11
Y1 - 1991/11
N2 - Various biotinylated lectins were used to characterize and semiquantitate glycoconjugate residues in the tubotympanum. Epithelial goblet cells were stained predominantly by WGA, LFA, SNA, RCA-I, Con-A, LCA, SBA, PHA-E, and UEA; this finding suggests they contain α-neuraminic acid, β-galactose, α-mannose, N-acetyl α-galactosamine, and α-fucose. Glandular mucous cells were stained predominantly by WGA, LFA, SNA, and RCA-I; this finding suggests that they contain α-neuraminic acid and β-galactose. The glandular serous cells were stained predominantly by Con-A, WGA, and LFA; this finding suggests that they produced α-mannose and α-neuraminic acid that represented serum-type glycoprotein. The positive staining of epithelial goblet cells and glandular mucous cells with PNA after neuraminidase digestion suggests that they produced mucin-type glycoproteins. The staining of the mucous blanket by WGA, LFA, SNA, RCA-I, LCA, PNA, SBA, PHA-E, and UEA suggests the presence of α-neuraminic acid, β-galactose, N-acetyl α-galactosamine, and α-fucose. The epithelial cell (nonsecretory) surface was stained largely by WGA, LFA, SNA, RCA-I, Con-A, and LCA; this finding suggests the presence of α-neuramimic acid, β-galactose, and α-mannose.
AB - Various biotinylated lectins were used to characterize and semiquantitate glycoconjugate residues in the tubotympanum. Epithelial goblet cells were stained predominantly by WGA, LFA, SNA, RCA-I, Con-A, LCA, SBA, PHA-E, and UEA; this finding suggests they contain α-neuraminic acid, β-galactose, α-mannose, N-acetyl α-galactosamine, and α-fucose. Glandular mucous cells were stained predominantly by WGA, LFA, SNA, and RCA-I; this finding suggests that they contain α-neuraminic acid and β-galactose. The glandular serous cells were stained predominantly by Con-A, WGA, and LFA; this finding suggests that they produced α-mannose and α-neuraminic acid that represented serum-type glycoprotein. The positive staining of epithelial goblet cells and glandular mucous cells with PNA after neuraminidase digestion suggests that they produced mucin-type glycoproteins. The staining of the mucous blanket by WGA, LFA, SNA, RCA-I, LCA, PNA, SBA, PHA-E, and UEA suggests the presence of α-neuraminic acid, β-galactose, N-acetyl α-galactosamine, and α-fucose. The epithelial cell (nonsecretory) surface was stained largely by WGA, LFA, SNA, RCA-I, Con-A, and LCA; this finding suggests the presence of α-neuramimic acid, β-galactose, and α-mannose.
KW - chinchilla
KW - eustachian tube
KW - glycoconjugates
KW - lectins
KW - middle ear
KW - neuraminidase
UR - http://www.scopus.com/inward/record.url?scp=0025824685&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025824685&partnerID=8YFLogxK
U2 - 10.1177/000348949110001114
DO - 10.1177/000348949110001114
M3 - Article
C2 - 1746830
AN - SCOPUS:0025824685
SN - 0003-4894
VL - 100
SP - 933
EP - 943
JO - Annals of Otology, Rhinology & Laryngology
JF - Annals of Otology, Rhinology & Laryngology
IS - 11
ER -