GLUT1-dependent glycolysis regulates exacerbation of fibrosis via AIM2 inflammasome activation

Soo Jung Cho, Jong Seok Moon, Kiichi Nikahira, Ha Seon Yun, Rebecca Harris, Kyung Sook Hong, Huarong Huang, Augustine M.K. Choi, Heather Stout-Delgado

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Background Idiopathic pulmonary fibrosis (IPF) is a rapidly progressive, fatal lung disease that affects older adults. One of the detrimental natural histories of IPF is acute exacerbation of IPF (AE-IPF), of which bacterial infection is reported to play an important role. However, the mechanism by which bacterial infection modulates the fibrotic response remains unclear. Objectives Altered glucose metabolism has been implicated in the pathogenesis of fibrotic lung diseases. We have previously demonstrated that glucose transporter 1 (GLUT1)-dependent glycolysis regulates fibrogenesis in a murine fibrosis model. To expand on these findings, we hypothesised that GLUT1-dependent glycolysis regulates acute exacerbation of lung fibrogenesis during bacterial infection via AIM2 inflammasome activation. Results In our current study, using a murine model of Streptococcus pneumoniae (S. pneumoniae) infection, we investigated the potential role of GLUT1 on mediating fibrotic responses to an acute exacerbation during bleomycin-induced fibrosis. The results of our current study illustrate that GLUT1 deficiency ameliorates S. pneumoniae-mediated exacerbation of lung fibrosis (wild type (WT)/phosphate buffered saline (PBS), n=3; WT/S. pneumoniae, n=3; WT/Bleomycin, n=5; WT/Bleomycin+S. pneumoniae, n=7; LysM-Cre-Glut1 fl/f/PBS, n=3; LysM-Cre-Glut1 fl/fl/S. pneumoniae, n=3; LysM-Cre-Glut1 fl/fl/Bleomycin, n=6; LysM-Cre-Glut1 fl/fl/Bleomycin+S. pneumoniae, n=9, p=0.041). Further, the AIM2 inflammasome, a multiprotein complex essential for sensing cytosolic bacterial DNA as a danger signal, is an important regulator of this GLUT1-mediated fibrosis and genetic deficiency of AIM2 reduced bleomycin-induced fibrosis after S. pneumoniae infection (WT/PBS, n=6; WT/Bleomycin+S. pneumoniae, n=15; Aim2-/-/PBS, n=6, Aim2-/-/Bleomycin+S. pneumoniae, n=11, p=0.034). GLUT1 deficiency reduced expression and function of the AIM2 inflammasome, and AIM2-deficient mice showed substantial reduction of lung fibrosis after S. pneumoniae infection. Conclusion Our results demonstrate that GLUT1-dependent glycolysis promotes exacerbation of lung fibrogenesis during S. pneumoniae infection via AIM2 inflammasome activation.

Original languageEnglish (US)
Pages (from-to)227-232
Number of pages6
JournalThorax
Volume75
Issue number3
DOIs
StatePublished - Mar 1 2020

Keywords

  • idiopathic pulmonary fibrosis
  • interstitial fibrosis
  • pneumonia

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Fingerprint Dive into the research topics of 'GLUT1-dependent glycolysis regulates exacerbation of fibrosis via AIM2 inflammasome activation'. Together they form a unique fingerprint.

Cite this