Glucocorticoid receptor interaction with Hsp90 remains unaltered after whole body hyperthermia

A. Cvoro, G. Matić

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The influence of 41°C whole body hyperthermic stress on glucocorticoid receptor (GR) association with 90-kDa heat shock protein (Hsp90) in the rat liver cytosol was examined. Total cytosolic GR and Hsp90 concentrations, as well as the amount of Hsp90 co-immunoprecipitated with the GR were determined by quantitative Western blotting using BuGR2 as anti-GR and AC88 as anti- Hsp90 monoclonal antibody. After exposure of the animals to the heat stress, the level of cytosolic Hsp90 increased, while its ratio to apo-receptor within non-activated GR heterooligomeric complexes remained unaltered. Therefore, the Hsp90 recruitment by the GR was not dependent on Hsp90 total cytosolic concentration.

Original languageEnglish (US)
Pages (from-to)257-260
Number of pages4
JournalStress
Volume3
Issue number3
DOIs
StatePublished - 2000

Keywords

  • Glucocorticoid receptor
  • Hsp90
  • Whole body hyperthermia

ASJC Scopus subject areas

  • Physiology
  • Neuropsychology and Physiological Psychology
  • Endocrine and Autonomic Systems
  • Psychiatry and Mental health
  • Behavioral Neuroscience

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