Glucocorticoid receptor inhibits microtubule assembly in vitro

Gunnar Akner; Ann Charlotte Wikström; Per Erik Strömstedt; Olov Stockman; Jan Åke Gustafsson; Margareta Wallin

doi:10.1016/0303-7207(95)03515-9

Glucocorticoid receptor inhibits microtubule assembly in vitro

Gunnar Akner, Ann Charlotte Wikström, Per Erik Strömstedt, Olov Stockman, Jan Åke Gustafsson, Margareta Wallin

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The effect of glucocorticoid hormones, purified glucocorticoid receptor (GR) and purified heat shock protein M_r 90 000 (hsp90) on microtubule (MT) assembly in vitro was tested by a spectrophotometric MT assembly assay and electron microscopy. GR significantly prolonged the nucleation phase, slowed down the assembly rate and reduced the maximal amplitude of MT assembly compared with control. The effects were partially reversed by the addition of glucocorticoid hormone. GR associated with MTs. These results indicate that GR affects MT assembly in vitro, which may be a functional correlate to the structural association of GR with MTs. This implies that factors affecting GR may affect MT assembly in vivo.

Original language	English (US)
Pages (from-to)	49-54
Number of pages	6
Journal	Molecular and cellular endocrinology
Volume	110
Issue number	1-2
DOIs	https://doi.org/10.1016/0303-7207(95)03515-9
State	Published - Apr 28 1995

Keywords

Co-localization
Glucocorticoid receptor
Microtubule assembly
Microtubules

ASJC Scopus subject areas

Molecular Biology
Endocrinology
Biochemistry
Endocrinology, Diabetes and Metabolism

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title = "Glucocorticoid receptor inhibits microtubule assembly in vitro",

abstract = "The effect of glucocorticoid hormones, purified glucocorticoid receptor (GR) and purified heat shock protein Mr 90 000 (hsp90) on microtubule (MT) assembly in vitro was tested by a spectrophotometric MT assembly assay and electron microscopy. GR significantly prolonged the nucleation phase, slowed down the assembly rate and reduced the maximal amplitude of MT assembly compared with control. The effects were partially reversed by the addition of glucocorticoid hormone. GR associated with MTs. These results indicate that GR affects MT assembly in vitro, which may be a functional correlate to the structural association of GR with MTs. This implies that factors affecting GR may affect MT assembly in vivo.",

keywords = "Co-localization, Glucocorticoid receptor, Microtubule assembly, Microtubules",

author = "Gunnar Akner and Wikstr{\"o}m, {Ann Charlotte} and Str{\"o}mstedt, {Per Erik} and Olov Stockman and Gustafsson, {Jan {\AA}ke} and Margareta Wallin",

note = "Funding Information: The authorsw ish to thankU lla Svedin for valuable help with the electronm icroscopice xaminationsT. he skillful work by Marika Ronnholmr egardingp urification of hsp90a ndp roductiono f monoclonaal nti-GRa nda nti-hsp90a ntibodiesis greatlya ppreciatedT.h is work was supportedb y grantsf rom the SwedishM edical Research Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

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doi = "10.1016/0303-7207(95)03515-9",

language = "English (US)",

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AU - Akner, Gunnar

AU - Wikström, Ann Charlotte

AU - Strömstedt, Per Erik

AU - Stockman, Olov

AU - Gustafsson, Jan Åke

AU - Wallin, Margareta

N1 - Funding Information: The authorsw ish to thankU lla Svedin for valuable help with the electronm icroscopice xaminationsT. he skillful work by Marika Ronnholmr egardingp urification of hsp90a ndp roductiono f monoclonaal nti-GRa nda nti-hsp90a ntibodiesis greatlya ppreciatedT.h is work was supportedb y grantsf rom the SwedishM edical Research Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1995/4/28

Y1 - 1995/4/28

N2 - The effect of glucocorticoid hormones, purified glucocorticoid receptor (GR) and purified heat shock protein Mr 90 000 (hsp90) on microtubule (MT) assembly in vitro was tested by a spectrophotometric MT assembly assay and electron microscopy. GR significantly prolonged the nucleation phase, slowed down the assembly rate and reduced the maximal amplitude of MT assembly compared with control. The effects were partially reversed by the addition of glucocorticoid hormone. GR associated with MTs. These results indicate that GR affects MT assembly in vitro, which may be a functional correlate to the structural association of GR with MTs. This implies that factors affecting GR may affect MT assembly in vivo.

AB - The effect of glucocorticoid hormones, purified glucocorticoid receptor (GR) and purified heat shock protein Mr 90 000 (hsp90) on microtubule (MT) assembly in vitro was tested by a spectrophotometric MT assembly assay and electron microscopy. GR significantly prolonged the nucleation phase, slowed down the assembly rate and reduced the maximal amplitude of MT assembly compared with control. The effects were partially reversed by the addition of glucocorticoid hormone. GR associated with MTs. These results indicate that GR affects MT assembly in vitro, which may be a functional correlate to the structural association of GR with MTs. This implies that factors affecting GR may affect MT assembly in vivo.

KW - Co-localization

KW - Glucocorticoid receptor

KW - Microtubule assembly

KW - Microtubules

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Glucocorticoid receptor inhibits microtubule assembly in vitro

Abstract

Keywords

ASJC Scopus subject areas

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