Glucocorticoid receptor inhibits microtubule assembly in vitro

Gunnar Akner; Ann Charlotte Wikström; Per Erik Strömstedt; Olov Stockman; Jan-Ake Gustafsson; Margareta Wallin

doi:10.1016/0303-7207(95)03515-9

Glucocorticoid receptor inhibits microtubule assembly in vitro

Gunnar Akner, Ann Charlotte Wikström, Per Erik Strömstedt, Olov Stockman, Jan-Ake Gustafsson, Margareta Wallin

Research output: Contribution to journal › Article

7 Scopus citations

Abstract

The effect of glucocorticoid hormones, purified glucocorticoid receptor (GR) and purified heat shock protein M_r 90 000 (hsp90) on microtubule (MT) assembly in vitro was tested by a spectrophotometric MT assembly assay and electron microscopy. GR significantly prolonged the nucleation phase, slowed down the assembly rate and reduced the maximal amplitude of MT assembly compared with control. The effects were partially reversed by the addition of glucocorticoid hormone. GR associated with MTs. These results indicate that GR affects MT assembly in vitro, which may be a functional correlate to the structural association of GR with MTs. This implies that factors affecting GR may affect MT assembly in vivo.

Original language	English (US)
Pages (from-to)	49-54
Number of pages	6
Journal	Molecular and cellular endocrinology
Volume	110
Issue number	1-2
DOIs	https://doi.org/10.1016/0303-7207(95)03515-9
State	Published - Apr 28 1995

Keywords

Co-localization
Glucocorticoid receptor
Microtubule assembly
Microtubules

ASJC Scopus subject areas

Molecular Biology
Endocrinology
Biochemistry
Endocrinology, Diabetes and Metabolism

Access to Document

10.1016/0303-7207(95)03515-9

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title = "Glucocorticoid receptor inhibits microtubule assembly in vitro",

abstract = "The effect of glucocorticoid hormones, purified glucocorticoid receptor (GR) and purified heat shock protein Mr 90 000 (hsp90) on microtubule (MT) assembly in vitro was tested by a spectrophotometric MT assembly assay and electron microscopy. GR significantly prolonged the nucleation phase, slowed down the assembly rate and reduced the maximal amplitude of MT assembly compared with control. The effects were partially reversed by the addition of glucocorticoid hormone. GR associated with MTs. These results indicate that GR affects MT assembly in vitro, which may be a functional correlate to the structural association of GR with MTs. This implies that factors affecting GR may affect MT assembly in vivo.",

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AU - Gustafsson, Jan-Ake

AU - Wallin, Margareta

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