Glucocorticoid receptor inhibits microtubule assembly in vitro

Gunnar Akner, Ann Charlotte Wikström, Per Erik Strömstedt, Olov Stockman, Jan Åke Gustafsson, Margareta Wallin

    Research output: Contribution to journalArticlepeer-review

    7 Scopus citations

    Abstract

    The effect of glucocorticoid hormones, purified glucocorticoid receptor (GR) and purified heat shock protein Mr 90 000 (hsp90) on microtubule (MT) assembly in vitro was tested by a spectrophotometric MT assembly assay and electron microscopy. GR significantly prolonged the nucleation phase, slowed down the assembly rate and reduced the maximal amplitude of MT assembly compared with control. The effects were partially reversed by the addition of glucocorticoid hormone. GR associated with MTs. These results indicate that GR affects MT assembly in vitro, which may be a functional correlate to the structural association of GR with MTs. This implies that factors affecting GR may affect MT assembly in vivo.

    Original languageEnglish (US)
    Pages (from-to)49-54
    Number of pages6
    JournalMolecular and cellular endocrinology
    Volume110
    Issue number1-2
    DOIs
    StatePublished - Apr 28 1995

    Keywords

    • Co-localization
    • Glucocorticoid receptor
    • Microtubule assembly
    • Microtubules

    ASJC Scopus subject areas

    • Molecular Biology
    • Endocrinology
    • Biochemistry
    • Endocrinology, Diabetes and Metabolism

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