TY - JOUR
T1 - Glioblastoma adhesion in a quick-fit hybrid microdevice
AU - Tsai, Hsieh Fu
AU - Toda-Peters, Kazumi
AU - Shen, Amy Q.
N1 - Funding Information:
Acknowledgements H.F.T is a JSPS DC1 fellow (Japan Society of Promotion for Science) and this work is supported by JSPS KAKENHI [Grant Number JP1700362]. The authors also thank Okinawa Institute of Science and Technology Graduate University (OIST) for its financial support with subsidy funding from the Cabinet Office, Government of Japan. Funders had no role in study design, data collection, the decision to publish, or preparation of the manuscript. The authors thank Ms. Fuka Koja from Image Analysis Section of OIST for technical assistance on flow cytometry. The authors thank Ms. Yi-Ching Tsai for her assistance on illustration preparation. The authors thank Dr. Steven D. Aird from OIST for the proofread.
Funding Information:
H.F.T is a JSPS DC1 fellow (Japan Society of Promotion for Science) and this work is supported by JSPS KAKENHI [Grant Number JP1700362]. The authors also thank Okinawa Institute of Science and Technology Graduate University (OIST) for its financial support with subsidy funding from the Cabinet Office, Government of Japan. Funders had no role in study design, data collection, the decision to publish, or preparation of the manuscript. The authors thank Ms. Fuka Koja from Image Analysis Section of OIST for technical assistance on flow cytometry. The authors thank Ms. Yi-Ching Tsai for her assistance on illustration preparation. The authors thank Dr. Steven D. Aird from OIST for the proofread.
Publisher Copyright:
© 2019, The Author(s).
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Translational research requires reliable biomedical microdevices (BMMD) to mimic physiological conditions and answer biological questions. In this work, we introduce a reversibly sealed quick-fit hybrid BMMD that is operator-friendly and bubble-free, requires low reagent and cell consumption, enables robust and high throughput performance for biomedical experiments. Specifically, we fabricate a quick-fit poly(methyl methacrylate) and poly(dimethyl siloxane) (PMMA/PDMS) prototype to illustrate its utilities by probing the adhesion of glioblastoma cells (T98G and U251MG) to primary endothelial cells. In static condition, we confirm that angiopoietin-Tie2 signaling increases the adhesion of glioblastoma cells to endothelial cells. Next, to mimic the physiological hemodynamic flow and investigate the effect of physiological electric field, the endothelial cells are pre-conditioned with concurrent shear flow (with fixed 1 Pa shear stress) and direct current electric field (dcEF) in the quick-fit PMMA/PDMS BMMD. With shear flow alone, endothelial cells exhibit classical parallel alignment; while under a concurrent dcEF, the cells align perpendicularly to the electric current when the dcEF is greater than 154 V m − 1 . Moreover, with fixed shear stress of 1 Pa, T98G glioblastoma cells demonstrate increased adhesion to endothelial cells conditioned in dcEF of 154 V m − 1 , while U251MG glioblastoma cells show no difference. The quick-fit hybrid BMMD provides a simple and flexible platform to create multiplex systems, making it possible to investigate complicated biological conditions for translational research.
AB - Translational research requires reliable biomedical microdevices (BMMD) to mimic physiological conditions and answer biological questions. In this work, we introduce a reversibly sealed quick-fit hybrid BMMD that is operator-friendly and bubble-free, requires low reagent and cell consumption, enables robust and high throughput performance for biomedical experiments. Specifically, we fabricate a quick-fit poly(methyl methacrylate) and poly(dimethyl siloxane) (PMMA/PDMS) prototype to illustrate its utilities by probing the adhesion of glioblastoma cells (T98G and U251MG) to primary endothelial cells. In static condition, we confirm that angiopoietin-Tie2 signaling increases the adhesion of glioblastoma cells to endothelial cells. Next, to mimic the physiological hemodynamic flow and investigate the effect of physiological electric field, the endothelial cells are pre-conditioned with concurrent shear flow (with fixed 1 Pa shear stress) and direct current electric field (dcEF) in the quick-fit PMMA/PDMS BMMD. With shear flow alone, endothelial cells exhibit classical parallel alignment; while under a concurrent dcEF, the cells align perpendicularly to the electric current when the dcEF is greater than 154 V m − 1 . Moreover, with fixed shear stress of 1 Pa, T98G glioblastoma cells demonstrate increased adhesion to endothelial cells conditioned in dcEF of 154 V m − 1 , while U251MG glioblastoma cells show no difference. The quick-fit hybrid BMMD provides a simple and flexible platform to create multiplex systems, making it possible to investigate complicated biological conditions for translational research.
KW - Bubble-free
KW - Electric field
KW - Endothelium
KW - Glioblastoma adhesion
KW - Multiplexing
KW - Shear flow
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UR - http://www.scopus.com/inward/citedby.url?scp=85063199025&partnerID=8YFLogxK
U2 - 10.1007/s10544-019-0382-0
DO - 10.1007/s10544-019-0382-0
M3 - Article
C2 - 30900024
AN - SCOPUS:85063199025
SN - 1387-2176
VL - 21
JO - Biomedical Microdevices
JF - Biomedical Microdevices
IS - 2
M1 - 30
ER -